Dalrd3 (NM_026378) Mouse Tagged ORF Clone

CAT#: MR220507

Dalrd3 (Myc-DDK-tagged) - Mouse DALR anticodon binding domain containing 3 (Dalrd3)

Product Images

Circular map for MR220507

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dalrd3
• Synonyms: 6330580J24Rik; C77829; mir-425
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220507 representing NM_026378
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGGGCGCCTTGGGGTTGGCGAGACGCTGGAGGCCCTTAATGCAGCTGTAGGGCCTGGTAGCC
  CAGTGTGGTTTAAGGAAACCCATGCCCGGCACCTTCGTGTTCGAGACTTCTTGGCACCGCGAAGCGCGCT
  ACAGGCGCGCTTCAGGGACGGCCAGGTGCCAGAGTGCGTGTTCCGTGCGGTCTCCTGCCTGCAGGGTCCC
  GGAGTGGCCCCTGTACTGCGCTGCGCGCCGACACCCGCGGGTTTATCGCTCCAACTACAGCGGCCCGCTG
  TCTTCGAACATGTACTCGGTGCCCTGGCCTCCTATGCTACTCCCGCCAAACCTGCCTCACCAGGCCCGCG
  AGTTGTCCTACACTGCCCAGCACTGCGCTGTAATTCGGGGACGCTCCGATTGAGCCAGCTGCGTGCCGTG
  CTCGTAGCCGATCATCTAGTACGGGTACTGCGTGCTCATGGGGTACGTGTGTGCTCAGTGCCCCCGGTGC
  GGGACCCTCACATGTCAACTTTCCTCCAGAAACTTCGGGTGGAATGGCCCACTGCCTCGAAGAGCACCTC
  GACCGAAACACTGAGAACCTGTGTTCTTGCAAACCTTAATGGTTCTAAGGAAGCGACGCTGCCCCCTGGC
  GTCCTAGGCCGACTGTGTCTGAAGGAACTGGTAGAACAGAGAGGCACTGCTGGCTATGACCCCAGCATAG
  ACCACTGTTTGGTGACTGAAGATGTGCTCTCTGTGCTCTCGGAGCTGCAGGAGGCTGTGCGCCACTGGCC
  AGAGGGCGGCCACCCAGGCCCGGCTGGGCGTCCAGATGCTGGTGTAGATGACTGCGTGGTTATACACGTG
  GTTAGCTGTGAAGAGGCATTCCAGCAACAAAAGCTGGATTTGCTCTGGCAGAAATTGGATGACCGGGCTC
  CTCACAAGCAGAAACACCTGGTGTGTGGTCCCGTGAAAATGGCTGGTGTACCCGGCACCCAGCTGACTGC
  CCCTCAGTACTACAGGCTCAGACATGCTCAGGTGTGCGAGGCCTCAGCTCTGAAGCACGGAGGAGACCTG
  GCACAAGACCCCGCCTGGACTGAGACCTTCGACATCCTCTCCGTGGCTACCATCAAATTTGAGATGCTAA
  GCACAGCCCCACAGAGCCAGCTCCTCCTGGCCCACAGCACCATCTCAACAAAGGGCACAAAGAGCGGGAC
  TTTTGTCATGTATAACTGTGCCCGCCTTGCCACACTCTTCGAGGGCTACAAACATGGAACGGAGCAAGGT
  CTGTATCCCACTTTTCCGCTTGTGAGCAGTCTGGACTTCTCACTACTCCATGATGAGGGTGAGTGGCTGC
  TGCTTTTCAACAGTGTCCTTCCCTTTCTGGACCTGCTGAGCCAGACTGTGAGTCTGGCCGGCACCCCAGG
  CCTCCACATCCCTGTCCGCACGGAGATGGTATGCAAGTTCCTGGTGCAGCTCAGCATGGATTTCAGCTCC
  TACTATAACCGAGTACATATTCTAGGGGAACCTCGGCCACACCTCTTTGGTCAGATGTTTGCTCGCCTAC
  AGCTTTTGAGGGCAGTGCGTGAGGTGTTCCACACAGGCTTGGCTATGCTGGGCCTCCCTCCGCTGAGCCA
  CATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220507 representing NM_026378
  Red=Cloning site Green=Tags(s)
  
  MATGRLGVGETLEALNAAVGPGSPVWFKETHARHLRVRDFLAPRSALQARFRDGQVPECVFRAVSCLQGP
  GVAPVLRCAPTPAGLSLQLQRPAVFEHVLGALASYATPAKPASPGPRVVLHCPALRCNSGTLRLSQLRAV
  LVADHLVRVLRAHGVRVCSVPPVRDPHMSTFLQKLRVEWPTASKSTSTETLRTCVLANLNGSKEATLPPG
  VLGRLCLKELVEQRGTAGYDPSIDHCLVTEDVLSVLSELQEAVRHWPEGGHPGPAGRPDAGVDDCVVIHV
  VSCEEAFQQQKLDLLWQKLDDRAPHKQKHLVCGPVKMAGVPGTQLTAPQYYRLRHAQVCEASALKHGGDL
  AQDPAWTETFDILSVATIKFEMLSTAPQSQLLLAHSTISTKGTKSGTFVMYNCARLATLFEGYKHGTEQG
  LYPTFPLVSSLDFSLLHDEGEWLLLFNSVLPFLDLLSQTVSLAGTPGLHIPVRTEMVCKFLVQLSMDFSS
  YYNRVHILGEPRPHLFGQMFARLQLLRAVREVFHTGLAMLGLPPLSHI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026378
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026378.3, NP_080654.3
• RefSeq Size: 1737 bp
• RefSeq ORF: 1617 bp
• Locus ID: 67789
• UniProt ID: Q6PJN8
• Cytogenetics: 9 F2
• MW: 59.2 kDa
• Gene Summary: Involved in tRNA methylation. Facilitates the recognition and targeting of tRNA(Arg)(CCU) and tRNA(Arg)(UCU) substrates for N(3)-methylcytidine modification by METTL2.[UniProtKB/Swiss-Prot Function]