Wdr4 (NM_021322) Mouse Tagged ORF Clone

CAT#: MR216848

Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (Wdr4)

Product Images

Circular map for MR216848

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr4
• Synonyms: AI415180; AI448349; D530049K22Rik; Wh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216848 representing NM_021322
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTACGCCCTGCGCGCATGCTCCTGGACGGCACGCCGTTCGCGCGGCGCCGAGTGACATCACTGA
  CAAGCGCCAACAGGAAGGGCGCAGCGCGTCGCACGTGTCCGGAGGCGGCGGGCGGGCCCATGGCGAGCTC
  TGCGGGGCTGGCACTGTGCGCCCAGACGCTGGTGGTGCGAGGAGGCAGCCGGTTCCTAGCCTTCTCCACT
  ACGGGCAGTGATGATGACTGTGTCTTCACATACGACTGCAGTACTGCAGAGAAGAAGGCCACGCCAGAAG
  ATAAAGGGGAGGACGGACAGCCCGCAGACACAGGGAGTGACTCGATTCTGGCGTCCACCTTCTCCAAGTC
  TGGCCGCTATTTTGCTTTAACAGATGACAGTAAGCGTCTGATTCTTTTCCGTACAAAACCATGGCAATGT
  CTGAGTGTCAGGATGGTGGTGCGGAGGTGCACCGCCCTGACCTTCACAGCCTCAGAGGACCGAGTCTTGG
  TGGCTGACAAGTCTGGAGACGTCTACTCCTTTTCGGTGCTGGAGCCAGATGGATGTGGCAGGCTGGAGCT
  TGGGCACCTCTCCATGCTGCTAGACGTGGCTGTGAGTCCTGATGACCAGTTTGTGCTTACTGCAGACCGG
  GATGAGAAGATCCGGGTCAGCTGGGCTGCTGCCCCGCATAGCATCGAGTCTTTCTGCCTGGGACACACTG
  AGTTTGTGAGCCGCATCCTTGTAGTGCCCAGTCATCCTGAACTGCTGCTTTCTTCCTCTGGGGATGGCAC
  CCTGAGACTCTGGGAGTACAGAAGCGGTAGGCAGCTGCAGTGCTGTGACCTGGCCGGCCTACAGGAGCCT
  GGAGAGCAGCCAGGCCACAAGGGGTTGGCCGCGTCCAGGATTGCATTCTGGGGACAGGAGAGCTATGTGG
  TGCTTCTGTGTGAGTGCGTTCCCGTGGTCTTCGTCTTCCAGCTTGATGCCAGCAGACAGCAGCTGGTGTT
  CAGACAGCGGCTGACTTTCCCTCACCGAGTGTGGGATGTTGTGTTTGAGGAGGCCCGGGGGCTGTGGGTT
  CTACAGGACTGCCGTGATGCCCCCCTGGTGCTCTGGAGGCCTGTGGGTGGTGAGTGGCAGGCTGCTCCAG
  ACGGTGCTGTGTCCCCGAGACTCTGCAGCCATCTCCGTGAGAGCTGGGCCATGCTGGAAGGTTCTGTTGG
  TACAGATGACAGCTTCCGCAGCCTGTACAAGGCCACCTTTGACAACATGACCTCTTACCTGAAGAAAAAG
  GAAGAGAGACTGCAGCAGCAGCTGAAGAAGAAGCGGCAAAGGAGCCCCTTCCCAGGGTCCCCGGAACAGA
  CCAAAAAGGCGTGCCCGGGCCAGTCAGCCCTTAGTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216848 representing NM_021322
  Red=Cloning site Green=Tags(s)
  
  MRLRPARMLLDGTPFARRRVTSLTSANRKGAARRTCPEAAGGPMASSAGLALCAQTLVVRGGSRFLAFST
  TGSDDDCVFTYDCSTAEKKATPEDKGEDGQPADTGSDSILASTFSKSGRYFALTDDSKRLILFRTKPWQC
  LSVRMVVRRCTALTFTASEDRVLVADKSGDVYSFSVLEPDGCGRLELGHLSMLLDVAVSPDDQFVLTADR
  DEKIRVSWAAAPHSIESFCLGHTEFVSRILVVPSHPELLLSSSGDGTLRLWEYRSGRQLQCCDLAGLQEP
  GEQPGHKGLAASRIAFWGQESYVVLLCECVPVVFVFQLDASRQQLVFRQRLTFPHRVWDVVFEEARGLWV
  LQDCRDAPLVLWRPVGGEWQAAPDGAVSPRLCSHLRESWAMLEGSVGTDDSFRSLYKATFDNMTSYLKKK
  EERLQQQLKKKRQRSPFPGSPEQTKKACPGQSALSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021322
• ORF Size: 1368 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021322.2, NP_067297.2
• RefSeq Size: 3906 bp
• RefSeq ORF: 1371 bp
• Locus ID: 57773
• UniProt ID: Q9EP82
• Cytogenetics: 17 B1
• MW: 50.9 kDa
• Gene Summary: Non-catalytic component of a methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320). In the methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit (By similarity). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (PubMed:29983320). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (By similarity). Also required for methylation of a specific subset of miRNAs, such as let-7 (By similarity). Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320). Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed:29574139).[UniProtKB/Swiss-Prot Function]