Grhl2 (NM_026496) Mouse Tagged ORF Clone

CAT#: MR209534

Grhl2 (Myc-DDK-tagged) - Mouse grainyhead-like 2 (Drosophila) (Grhl2)

Product Images

Circular map for MR209534

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grhl2
• Synonyms: 0610015A08Rik; BOM; clft3; Tcfcp2l3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209534 representing NM_026496
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACAAGAGTCGGACAATAATAAAAGACTAGTGGCCTTAGTGCCCATGCCCAGTGACCCTCCCTTCA
  ACACCCGAAGAGCCTACACAAGTGAGGATGAGGCCTGGAAGTCATATCTGGAGAACCCCCTGACTGCGGC
  CACCAAGGCGATGATGAGCATCAACGGGGACGAGGACAGTGCTGCCGCCCTGGGCCTGCTCTATGACTAC
  TACAAGGTTCCTCGAGACAAGAGACTTCTGTCTGTGAGCAAAGCAAGTGACAGCCAAGAAGACCAGGATA
  AAAGAAACTGCCTTGGCACCAGTGAAGCCCAGATCAATTTGAGCGGAGGCGAGAACAGAGTGCAGGTTCT
  GAAGACTGTCCCGGTGAACCTCTGTCTAAGTCAAGACCACATGGAGAATTCGAAGCGCGAGCAGTACAGT
  GTATCCATCACCGAGAGCTCTGCCGTCATCCCCGTGTCAGGCATCACCGTGGTGAAAGCCGAGGATTTCA
  CACCGGTGTTCATGGCGCCCCCGGTGCACTATCCCCGCGCGGACAGTGAGGAGCAGCGCGTGGTTATCTT
  TGAACAGACTCAGTACGACCTGCCCTCCATAGCCAGCCACAGCTCCTATCTCAAGGACGACCAGCGCAGC
  ACGCCGGACAGCACCTACAGCGAGAGCTTTAAGGACGGCGCCTCGGAGAAATTTCGGAGTACTTCTGTTG
  GTGCTGACGAGTATACATATGACCAGACGGGAAGTGGTACATTTCAGTACACCCTGGAAGCCACCAAATC
  TCTCCGTCAGAAGCAGGGGGAGGGCCCCATGACCTACCTCAACAAAGGACAATTCTATGCCATAACACTC
  AGTGAGACTGGAGACAACAAATGCTTCCGACACCCCATCAGCAAAGTCAGGAGTGTGGTGATGGTGGTCT
  TTAGTGAAGACAAAAACCGAGATGAGCAGCTGAAATACTGGAAGTACTGGCACTCCCGGCAGCACACTGC
  CAAGCAGAGGGTCCTTGACATTGCTGATTACAAGGAGAGCTTCAACACCATCGGGAACATTGAAGAGATC
  GCATACAATGCTGTTTCCTTCACCTGGGATGTGAACGAGGAGGCAAAGATTTTTATCACCGTGAATTGCC
  TGAGTACAGATTTCTCCTCCCAAAAGGGTGTAAAAGGACTTCCCCTGATGATTCAGATCGACACGTACAG
  CTACAACAACCGCAGCAATAAACCCATCCACAGAGCATACTGCCAGATCAAGGTCTTCTGTGACAAGGGA
  GCAGAAAGAAAAATCCGGGATGAAGAGAGAAAGCAGAACAGGAAGAAAGGGAAGGGCCAGGCCTCTCAAG
  CCCAGTGCAACAACTCCTCTGATGGGAAGATGGCCGCCATACCGTTACAGAAGAAGAGTGACATCACGTA
  CTTCAAAACCATGCCCGACCTGCACTCACAGCCTGTGCTCTTCATACCAGATGTTCACTTTGCAAACCTA
  CAGAGGACCGGACAGGTTTATTACAACACAGACGATGAGCGAGAAGGCAGCAGCGTCCTTGTTAAGCGGA
  TGTTCAGGCCCATGGAAGAGGAGTTTGGTCCAACACCGTCTAAGCAGATCAAAGAAGAAAACGTAAAACG
  AGTGCTTTTATATGTGAGGAAGGAGAACGATGACGTCTTCGATGCTCTGATGCTGAAATCACCCACGGTG
  AAGGGTCTGATGGAAGCGCTGTCTGAGAAGTATGGGCTGCCAGTGGAGAAAATCACAAAGCTTTATAAGA
  AGAGCAAAAAGGGCATCCTGGTCAACATGGATGACAACATCATTGAGCACTATTCAAATGAGGACACCTT
  CATCCTCAACATGGAGAGCATGGTGGAAGGCTTCAAGATCACGCTGATGGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209534 representing NM_026496
  Red=Cloning site Green=Tags(s)
  
  MSQESDNNKRLVALVPMPSDPPFNTRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEDSAAALGLLYDY
  YKVPRDKRLLSVSKASDSQEDQDKRNCLGTSEAQINLSGGENRVQVLKTVPVNLCLSQDHMENSKREQYS
  VSITESSAVIPVSGITVVKAEDFTPVFMAPPVHYPRADSEEQRVVIFEQTQYDLPSIASHSSYLKDDQRS
  TPDSTYSESFKDGASEKFRSTSVGADEYTYDQTGSGTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITL
  SETGDNKCFRHPISKVRSVVMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEI
  AYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKG
  AERKIRDEERKQNRKKGKGQASQAQCNNSSDGKMAAIPLQKKSDITYFKTMPDLHSQPVLFIPDVHFANL
  QRTGQVYYNTDDEREGSSVLVKRMFRPMEEEFGPTPSKQIKEENVKRVLLYVRKENDDVFDALMLKSPTV
  KGLMEALSEKYGLPVEKITKLYKKSKKGILVNMDDNIIEHYSNEDTFILNMESMVEGFKITLMEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026496
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026496.4
• RefSeq Size: 4751 bp
• RefSeq ORF: 1878 bp
• Locus ID: 252973
• UniProt ID: Q8K5C0
• Cytogenetics: 15 B3.1
• MW: 71.6 kDa
• Gene Summary: Transcription factor playing an important role in primary neurulation and in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (PubMed:22696678). During embryogenesis, plays unique and cooperative roles with GRHL3 in establishing distinct zones of primary neurulation. Essential for closure 3 (rostral end of the forebrain), functions cooperatively with GRHL3 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (PubMed:20654612). Regulates epithelial morphogenesis acting as a target gene-associated transcriptional activator of apical junctional complex components. Up-regulates of CLDN3 and CLDN4, as well as of RAB25, which increases the CLDN4 protein and its localization at tight junctions (PubMed:22696678). Comprises an essential component of the transcriptional machinery that establishes appropriate expression levels of CLDN4 and CDH1 in different types of epithelia (PubMed:20978075). Exhibits functional redundancy with GRHL3 in epidermal morphogenetic events such as eyelid fusion and epidermal wound repair (PubMed:21081122). In lung, forms a regulatory loop with NKX2-1 that coordinates lung epithelial cell morphogenesis and differentiation (PubMed:22955271). In keratinocytes, plays a role in telomerase activation during cellular proliferation, regulates TERT expression by binding to TERT promoter region and inhibiting DNA methylation at the 5'-CpG island, possibly by interfering with DNMT1 enzyme activity. In addition, impairs keratinocyte differentiation and epidermal function by inhibiting the expression of genes clustered at the epidermal differentiation complex (EDC) as well as GRHL1 and GRHL3 through epigenetic mechanisms (By similarity).[UniProtKB/Swiss-Prot Function]