Wdr4 (BC039272) Mouse Tagged ORF Clone

CAT#: MR206503

Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)

Product Images

Circular map for MR206503

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr4
• Synonyms: AI415180; AI448349; D530049K22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206503 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCTCTGCGGGGCTGGCACTGTGCGCCCAGACGCTGGTGGTGCGAGGAGGCAGCCGGTTCCTAG
  CCTTCTCCACTACGGGCAGTGATGATGACTGTGTCTTCACATACGACTGCAGTACTGCAGAGAAGAAGGC
  CACGCCAGAAGATAAAGGGGAGGACGGACAGCCCGCAGACACAGGGAGTGACTCGATTCTGGCGTCCACC
  TTCTCCAAGTCTGGCCGCTATTTTGCTTTAACAGATGACAGTAAGCGTCTGATTCTTTTCCGTACAAAAC
  CATGGCAATGTCTGAGTGTCAGGATGGTGGTGCGGAGGTGCACCGCCCTGACCTTCACAGCCTCAGAGGA
  CCGAGTCTTGGTGGCTGACAAGTCTGGAGACGTCTACTCCTTTTCGGTGCTGGAGCCAGATGGATGTGGC
  AGGCTGGAGCTTGGGCACCTCTCCATGCTGCTAGACGTGGCTGTGAGTCCTGATGACCAGTTTGTGCTTA
  CTGCAGACCGGGATGAGAAGATCCGGGTCAGCTGGGCTGCTGCCCCGCATAGCATCGAGTCTTTCTGCCT
  GGGACACACTGAGTTTGTGAGCCGCATCCTTGTAGTGCCCAGTCATCCTGAACTGCTGCTTTCTTCCTCT
  GGGGATGGCACCCTGAGACTCTGGGAGTACAGAAGCGGTAGGCAGCTGCAGTGCTGTGACCTGGCCGGCC
  TACAGGAGCCTGGAGAGCAGCCAGGCCACAAGGGGTTGGCCGCGTCCAGGATTGCATTCTGGGGACAGGA
  GAGCTATGTGGTGCTTCTGTGTGAGTGCGTTCCCGTGGTCTTCGTCTTCCAGCTTGATGCCAGCAGACAG
  CAGCTGGTGTTCAGACAGCGGCTGACTTTCCCTCACCGAGTGTGGGATGTTGTGTTTGAGGAGGCCCGGG
  GGCTGTGGGTTCTACAGGACTGCCGTGATGCCCCCCTGGTGCTCTGGAGGCCTGTGGGTGGTGAGTGGCA
  GGCTGCTCCAGACGGTGCTGTGTCCCCGAGACTCTGCAGCCATCTCCGTGAGAGCTGGGCCATGCTGGAA
  GGTTCTGTTGGTACAGATGACAGCTTCCGCAGCCTGTACAAGGCCACCTTTGACAACATGACCTCTTACC
  TGAAGAAAAAGGAAGAGAGACTGCAGCAGCAGCTGAAGAAGAAGCGGCAAAGGAGCCCCTTCCCAGGGTC
  CCCGGAACAGACCAAAAAGGCGTGCCCGGGCCAGTCAGCCCTTAGTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206503 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASSAGLALCAQTLVVRGGSRFLAFSTTGSDDDCVFTYDCSTAEKKATPEDKGEDGQPADTGSDSILAST
  FSKSGRYFALTDDSKRLILFRTKPWQCLSVRMVVRRCTALTFTASEDRVLVADKSGDVYSFSVLEPDGCG
  RLELGHLSMLLDVAVSPDDQFVLTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRILVVPSHPELLLSSS
  GDGTLRLWEYRSGRQLQCCDLAGLQEPGEQPGHKGLAASRIAFWGQESYVVLLCECVPVVFVFQLDASRQ
  QLVFRQRLTFPHRVWDVVFEEARGLWVLQDCRDAPLVLWRPVGGEWQAAPDGAVSPRLCSHLRESWAMLE
  GSVGTDDSFRSLYKATFDNMTSYLKKKEERLQQQLKKKRQRSPFPGSPEQTKKACPGQSALSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC039272
• ORF Size: 1239 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC039272, AAH39272
• RefSeq Size: 2054 bp
• RefSeq ORF: 1241 bp
• Locus ID: 57773
• Cytogenetics: 17 B1
• MW: 45.8 kDa
• Gene Summary: Non-catalytic component of a methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320). In the methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit (By similarity). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (PubMed:29983320). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (By similarity). Also required for methylation of a specific subset of miRNAs, such as let-7 (By similarity). Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320). Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed:29574139).[UniProtKB/Swiss-Prot Function]