Rab26 (NM_177375) Mouse Tagged ORF Clone

CAT#: MR203367

Rab26 (Myc-DDK-tagged) - Mouse RAB26, member RAS oncogene family (Rab26)

Product Images

Circular map for MR203367

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rab26
• Synonyms: A830020M03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203367 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGGAAGAAGACCCCCAAGAGCAAGGGGGGGAGTGAGCCGGCTACCTCTACACTGCCCGCCGCCG
  CCGCCGCCACCAATGGGCCACGTCTGGCACATCCTAGGACTGTGCGCCCTGGCCCAGAGGCACCGCCCAA
  TGGGCCCCCGCAGTCCATCCGGCCCTCACTTGGTAGCACTGGAGACTTCTACGATGTTGCTTTCAAGGTC
  ATGCTGGTGGGGGATTCCGGTGTGGGGAAGACCTGCCTGCTTGTGCGCTTCAAGGATGGGGCTTTCCTGG
  CTGGTACCTTCATCTCCACTGTGGGCATCGACTTCCGGAATAAAGTTCTGGATGTGGATGGCATGAAGGT
  GAAGCTGCAGATCTGGGACACAGCTGGTCAGGAGCGGTTCCGAAGTGTCACCCATGCCTACTACCGAGAT
  GCTCACGCACTGTTGCTGCTCTACGACATCACCAACAAAGATTCCTTCGACAACATTCAGGCCTGGTTGA
  CAGAGATCCAGGAATATGCCCAGCAGGACGTGGTGCTCATGCTGCTAGGGAACAAGGTTGACTCTACTCA
  AGACCGTGTGGTAAAGAGGGAAGATGGGGAGAAATTAGCCAAGGAGTATGGGCTACCTTTCATGGAGACC
  AGCGCAAGGACTGGCCTCAACGTGGACTTGGCTTTTACAGCCATAGCAAAGGAGCTGAAACAGAGATCAG
  CCAAGGCTCCCAGCGAGCCCCGCTTCAGGCTGCATGACTATGTTAAGAGGGAGGGCCGAGGGGTCTCCTG
  CTGTCGACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203367 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRKKTPKSKGGSEPATSTLPAAAAATNGPRLAHPRTVRPGPEAPPNGPPQSIRPSLGSTGDFYDVAFKV
  MLVGDSGVGKTCLLVRFKDGAFLAGTFISTVGIDFRNKVLDVDGMKVKLQIWDTAGQERFRSVTHAYYRD
  AHALLLLYDITNKDSFDNIQAWLTEIQEYAQQDVVLMLLGNKVDSTQDRVVKREDGEKLAKEYGLPFMET
  SARTGLNVDLAFTAIAKELKQRSAKAPSEPRFRLHDYVKREGRGVSCCRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177375
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177375.1, NP_796349.1
• RefSeq Size: 1434 bp
• RefSeq ORF: 783 bp
• Locus ID: 328778
• UniProt ID: Q504M8
• Cytogenetics: 17 A3.3
• MW: 28.6 kDa
• Gene Summary: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Mediates transport of ADRA2A and ADRA2B from the Golgi to the cell membrane. Plays a role in the maturation of zymogenic granules and in pepsinogen secretion in the stomach (By similarity). Plays a role in the secretion of amylase from acinar granules in the parotid gland.[UniProtKB/Swiss-Prot Function]