Cdc42ep5 (NM_021454) Mouse Tagged ORF Clone
CAT#: MR201066
- TrueORF®
Cdc42ep5 (Myc-DDK-tagged) - Mouse CDC42 effector protein (Rho GTPase binding) 5 (Cdc42ep5)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_021454" in other vectors (3)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Cdc42ep5 |
Synonyms | 1700027J19Rik; 2010007O02Rik; Borg3; C85526; CEP5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201066 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTAATGAAGCAGCTGGGCCCTGCACAACCCAAGAAGCGGCTGGATCGCGGAGCACTCTCGATCT CAGCACCCCTCGGAGACTTCAGGCACACATTGCACGTGGGACGCGGTGGCGACGCCTTCGGGGACACCTC ATTCCTGAGTCGCCACGGGGGCGGTCCGCCTCCCGAGCCTGGAGCCCCACCGGTTGTGGCCCCACATTCT GTCGCTCCACCTGCAGCCCCACAGCCCCCTGTGGCTGTGCCTTCACCTGCGGACCCGCTGCTGTCTTTCC ACCTGGACCTGGGCCCCTCTATGCTGGACGCGGTGTTAGGCGTCATGGACGCGGAGCGCAGCGAGACAAC AGCCACCAAGCCCGATGGGGATGCCCACCCTAGAGTGCAGCACCCCAAGACCCGCTGCTGCTCAAATGCT GACCTCCAGCTGGACGACGTCATCGGTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201066 protein sequence
Red=Cloning site Green=Tags(s) MPVMKQLGPAQPKKRLDRGALSISAPLGDFRHTLHVGRGGDAFGDTSFLSRHGGGPPPEPGAPPVVAPHS VAPPAAPQPPVAVPSPADPLLSFHLDLGPSMLDAVLGVMDAERSETTATKPDGDAHPRVQHPKTRCCSNA DLQLDDVIGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021454 |
ORF Size | 453 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021454.4 |
RefSeq Size | 867 bp |
RefSeq ORF | 453 bp |
Locus ID | 58804 |
UniProt ID | Q9Z0X0 |
Cytogenetics | 7 A1 |
MW | 15.5 kDa |
Gene Summary | Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Inhibits MAPK8 independently of CDC42 binding. Controls septin organization and this effect is negatively regulated by CDC42.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG201066 | Cdc42ep5 (tGFP-tagged) - Mouse CDC42 effector protein (Rho GTPase binding) 5 (Cdc42ep5) |
USD 350.00 |
|
MR201066L3 | Lenti ORF clone of Cdc42ep5 (Myc-DDK-tagged) - Mouse CDC42 effector protein (Rho GTPase binding) 5 (Cdc42ep5) |
USD 450.00 |
|
MR201066L4 | Lenti ORF clone of Cdc42ep5 (mGFP-tagged) - Mouse CDC42 effector protein (Rho GTPase binding) 5 (Cdc42ep5) |
USD 450.00 |
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