Tlr2 (NM_011905) Mouse Tagged ORF Clone

CAT#: MG227674

Tlr2 (tGFP-tagged) - Mouse toll-like receptor 2 (Tlr2), (10ug)

Product Images

Circular map for MG227674

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tlr2
• Synonyms: Ly105
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227674 representing NM_011905
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACGAGCTCTTTGGCTCTTCTGGATCTTGGTGGCCATAACAGTCCTCTTCAGCAAACGCTGTTCTG
  CTCAGGAGTCTCTGTCATGTGATGCTTCTGGGGTGTGTGATGGCCGCTCCAGGTCTTTCACCTCTATTCC
  CTCCGGACTCACAGCAGCCATGAAAAGCCTTGACCTGTCTTTCAACAAGATCACCTACATTGGCCATGGT
  GACCTCCGAGCGTGTGCGAACCTCCAGGTTCTGATTTTGAAGTCCAGCAGAATCAATACAATAGAGGGAG
  ACGCCTTTTATTCTCTGGGCAGTCTTGAACATTTGGATTTGTCTGATAATCACCTATCTAGTTTATCTTC
  CTCCTGGTTCGGGCCCCTTTCCTCTTTGAAATACTTAAACTTAATGGGAAATCCTTACCAGACACTGGGG
  GTAACATCGCTTTTTCCCAATCTCACAAATTTACAAACCCTCAGGATAGGAAATGTAGAGACTTTCAGTG
  AGATAAGGAGAATAGATTTTGCTGGGCTGACTTCTCTCAATGAACTTGAAATTAAGGCATTAAGTCTCCG
  GAATTATCAGTCCCAAAGTCTAAAGTCGATCCGCGACATCCATCACCTGACTCTTCACTTAAGCGAGTCT
  GCTTTCCTGCTGGAGATTTTTGCAGATATTCTGAGTTCTGTGAGATATTTAGAACTAAGAGATACTAACT
  TGGCCAGGTTCCAGTTTTCACCACTGCCCGTAGATGAAGTCAGCTCACCGATGAAGAAGCTGGCATTCCG
  AGGCTCGGTTCTCACTGATGAAAGCTTTAACGAGCTCCTGAAGCTGTTGCGTTACATCTTGGAACTGTCG
  GAGGTAGAGTTCGACGACTGTACCCTCAATGGGCTCGGCGATTTCAACCCCTCGGAGTCAGACGTAGTGA
  GCGAGCTGGGTAAAGTAGAAACAGTCACTATCCGGAGGTTGCATATCCCCCAGTTCTATTTGTTTTATGA
  CCTGAGTACTGTCTATTCCCTCCTGGAGAAGGTGAAGCGAATCACAGTAGAGAACAGCAAGGTCTTCCTG
  GTTCCCTGCTCGTTCTCCCAGCATTTAAAATCATTAGAATTCTTAGACCTCAGCGAAAATCTGATGGTTG
  AAGAATATTTGAAGAACTCAGCCTGTAAGGGAGCCTGGCCTCCTCTACAAACCTTAGTTTTGAGCCAGAA
  TCATTTGAGATCAATGCAAAAAACAGGAGAGATTTTGCTGACTCTGAAAAACCTGACCTCCCTTGACATC
  AGCAGGAACACTTTTCATCCGATGCCCGACAGCTGTCAGTGGCCAGAAAAGATGCGCTTCCTGAATTTGT
  CCAGTACAGGGATCCGGGTGGTAAAAACGTGCATTCCTCAGACGCTGGAGGTGTTGGATGTTAGTAACAA
  CAATCTTGACTCATTTTCTTTGTTCTTGCCTCGGCTGCAAGAGCTCTATATTTCCAGAAATAAGCTGAAA
  ACACTCCCAGATGCTTCGTTGTTCCCTGTGTTGCTGGTCATGAAAATCAGAGAGAATGCAGTAAGTACTT
  TCTCTAAAGACCAACTTGGTTCTTTTCCCAAACTGGAGACTCTGGAAGCAGGCGACAACCACTTTGTTTG
  CTCCTGCGAACTCCTATCCTTTACTATGGAGACGCCAGCTCTGGCTCAAATCCTGGTTGACTGGCCAGAC
  AGCTACCTGTGTGACTCTCCGCCTCGCCTGCACGGCCACAGGCTTCAGGATGCCCGGCCCTCCGTCTTGG
  AATGTCACCAGGCTGCACTGGTGTCTGGAGTCTGCTGTGCCCTTCTCCTGTTGATCTTGCTCGTAGGTGC
  CCTGTGCCACCATTTCCACGGGCTGTGGTACCTGAGAATGATGTGGGCGTGGCTCCAGGCCAAGAGGAAG
  CCCAAGAAAGCTCCCTGCAGGGACGTTTGCTATGATGCCTTTGTTTCCTACAGTGAGCAGGATTCCCATT
  GGGTGGAGAACCTCATGGTCCAGCAGCTGGAGAACTCTGACCCGCCCTTTAAGCTGTGTCTCCACAAGCG
  GGACTTCGTTCCGGGCAAATGGATCATTGACAACATCATCGATTCCATCGAAAAGAGCCACAAAACTGTG
  TTCGTGCTTTCTGAGAACTTCGTACGGAGCGAGTGGTGCAAGTACGAACTGGACTTCTCCCACTTCAGGC
  TCTTTGACGAGAACAACGACGCGGCCATCCTTGTTTTGCTGGAGCCCATTGAGAGGAAAGCCATTCCCCA
  GCGCTTCTGCAAACTGCGCAAGATAATGAACACCAAGACCTACCTGGAGTGGCCCTTGGATGAAGGCCAG
  CAGGAAGTGTTTTGGGTAAATCTGAGAACTGCAATAAAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227674 representing NM_011905
  Red=Cloning site Green=Tags(s)
  
  MLRALWLFWILVAITVLFSKRCSAQESLSCDASGVCDGRSRSFTSIPSGLTAAMKSLDLSFNKITYIGHG
  DLRACANLQVLILKSSRINTIEGDAFYSLGSLEHLDLSDNHLSSLSSSWFGPLSSLKYLNLMGNPYQTLG
  VTSLFPNLTNLQTLRIGNVETFSEIRRIDFAGLTSLNELEIKALSLRNYQSQSLKSIRDIHHLTLHLSES
  AFLLEIFADILSSVRYLELRDTNLARFQFSPLPVDEVSSPMKKLAFRGSVLTDESFNELLKLLRYILELS
  EVEFDDCTLNGLGDFNPSESDVVSELGKVETVTIRRLHIPQFYLFYDLSTVYSLLEKVKRITVENSKVFL
  VPCSFSQHLKSLEFLDLSENLMVEEYLKNSACKGAWPPLQTLVLSQNHLRSMQKTGEILLTLKNLTSLDI
  SRNTFHPMPDSCQWPEKMRFLNLSSTGIRVVKTCIPQTLEVLDVSNNNLDSFSLFLPRLQELYISRNKLK
  TLPDASLFPVLLVMKIRENAVSTFSKDQLGSFPKLETLEAGDNHFVCSCELLSFTMETPALAQILVDWPD
  SYLCDSPPRLHGHRLQDARPSVLECHQAALVSGVCCALLLLILLVGALCHHFHGLWYLRMMWAWLQAKRK
  PKKAPCRDVCYDAFVSYSEQDSHWVENLMVQQLENSDPPFKLCLHKRDFVPGKWIIDNIIDSIEKSHKTV
  FVLSENFVRSEWCKYELDFSHFRLFDENNDAAILVLLEPIERKAIPQRFCKLRKIMNTKTYLEWPLDEGQ
  QEVFWVNLRTAIKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011905
• ORF Size: 2352 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011905.2
• RefSeq Size: 2874 bp
• RefSeq ORF: 2355 bp
• Locus ID: 24088
• UniProt ID: Q9QUN7
• Cytogenetics: 3 E3
• Gene Summary: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity) (PubMed:15690042). May also promote apoptosis in response to lipoproteins (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (By similarity). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (PubMed:17486091). Acts as the major receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PhoS1 (pstS1), in conjunction with TLR1 and for some but not all lipoproteins CD14 and/or CD36. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). Recombinant MPT83 from M.tuberculosis stimulates secretion of cytokines (TNF-alpha, IL-6 and IL-12p40) by mouse macrophage cell lines in a TLR2-dependent fashion, which leads to increased host innate immunity responses against the bacterium (PubMed:22174456). Lung macrophages which express low levels of TLR2 respond poorly to stimulation by M.tuberculosis LpqH (PubMed:19362712). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (PubMed:27220037). Interacts with TICAM2 (By similarity).[UniProtKB/Swiss-Prot Function]