Dusp16 (NM_001048054) Mouse Tagged ORF Clone

CAT#: MG225585

Dusp16 (tGFP-tagged) - Mouse dual specificity phosphatase 16 (Dusp16) transcript variant B1, (10ug)

Product Images

Circular map for MG225585

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dusp16
• Synonyms: 3830417M17Rik; AW558566; D6Ertd213e; MKP-7; MKP7; Mkpm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225585 representing NM_001048054
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCATGAGATGATTGGAACTCAAATTGTTACTGAGAGCTTGGTGGCTCTGCTGGAAAGTGGAACGG
  AAAAAGTGCTGCTAATTGATAGCCGACCATTTGTGGAATACAATACGTCTCACATTTTGGAAGCCATTAA
  TATCAACTGCTCCAAACTGATGAAGCGAAGGTTGCAACAGGACAAAGTATTAATTACAGAACTTATCCAA
  CATTCTGCAAAGCATAAGGTTGACATTGACTGCAATCAGAAGGTGGTAGTTTATGATCAAAGTTCCCAAG
  ATGTTGGTTCTCTGTCGTCAGACTGCTTTCTCACTGTACTTCTGGGTAAACTGGAGAGGAGCTTCAACTC
  TGTCCACCTGCTTGCAGGTGGCTTTGCTGAGTTCTCTCGTTGTTTCCCTGGCCTCTGTGAAGGAAAGTCC
  ACTCTAGTCCCTACCTGCATATCTCAGCCTTGCTTACCTGTTGCGAACATTGGGCCAACTCGAATTCTTC
  CCAATCTCTATCTTGGCTGCCAGCGAGATGTCCTCAACAAGGACCTGATGCAACAGAATGGGATTGGCTA
  TGTGTTAAATGCCAGCAATACCTGTCCAAAGCCTGACTTCATACCTGAATCTCACTTCCTGCGAGTGCCT
  GTGAATGACAGCTTTTGTGAGAAAATCCTACCATGGTTGGACAAGTCTGTGGATTTCATTGAGAAAGCAA
  AAGCCTCCAATGGCTGTGTGCTTATCCACTGCTTAGCTGGGATCTCTCGCTCCGCCACTATTGCTATTGC
  CTACATCATGAAGAGGATGGACATGTCTCTAGATGAGGCTTACAGGCGGCAGAAGCCTACTGACAGAGCT
  GACTCGAGGCGGAGCTGGCATGAAGAGAGCCCCTTTGAAAAGCAGTTTAAACGCAGAAGCTGCCAAATGG
  AATTTGGAGAGAGCATTATGTCGGAGAACAGGTCCAGGGAGGAGCTGGGCAAGGTGGGCAGCCAGTCCAG
  CTTCTCCGGCAGCATGGAGATCATCGAGGTCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225585 representing NM_001048054
  Red=Cloning site Green=Tags(s)
  
  MAHEMIGTQIVTESLVALLESGTEKVLLIDSRPFVEYNTSHILEAININCSKLMKRRLQQDKVLITELIQ
  HSAKHKVDIDCNQKVVVYDQSSQDVGSLSSDCFLTVLLGKLERSFNSVHLLAGGFAEFSRCFPGLCEGKS
  TLVPTCISQPCLPVANIGPTRILPNLYLGCQRDVLNKDLMQQNGIGYVLNASNTCPKPDFIPESHFLRVP
  VNDSFCEKILPWLDKSVDFIEKAKASNGCVLIHCLAGISRSATIAIAYIMKRMDMSLDEAYRRQKPTDRA
  DSRRSWHEESPFEKQFKRRSCQMEFGESIMSENRSREELGKVGSQSSFSGSMEIIEVS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001048054
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048054.2
• RefSeq Size: 4158 bp
• RefSeq ORF: 1017 bp
• Locus ID: 70686
• Cytogenetics: 6 65.77 cM