Aldh3b2 (NM_001177438) Mouse Tagged ORF Clone

CAT#: MG224974

Aldh3b2 (tGFP-tagged) - Mouse aldehyde dehydrogenase 3 family member B2 (Aldh3b2), (10ug)

Product Images

Circular map for MG224974

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aldh3b2
• Synonyms: AI848594; C130048D07Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224974 representing NM_001177438
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCTGCAGAGACAGGCTCGGAGCCCTCTCAGGGGGCAGGCCCCTCTGAGGCCACACTGCACAGCC
  TGCGGGAGGCCTTCAATGCAGGGCGGACTCGGCCTACTGAGTTTCGGACTGCACAGCTGCGGAGCCTGGG
  TCGCTTCTTGCAGGAAAACAAAGAGCTGCTGCAAGATGCACTGGCTAAGGACGTGGGCAAGTCAGGCTTC
  GAGTCAGACATGAGTGAGATCATCCTGTGCGAGAATGAGGTGGACCTGGCCCTCAAGAACCTGCAGACCT
  GGATGAAGGACGAGCCTGTGTCCACCAACTTGCTCACCAAGCTGAGCTCAGCCTTCATCCGGAAAGAGCC
  CTTTGGCCTGGTGCTTATCATTGCACCCTGGAATTATCCTGTGAACTTGATGATCATACCTTTAGTGGGG
  GCCATTGCTGCAGGAAACTGTGTGGTGCTGAAGCCATCAGAAATTAGCAAGAACACAGAGAAGGTGCTGG
  CAGAGCTGCTGCCCCAGTATCTGGACCAGAGCTGCTTTGCTGTGATGTTGGGAGGACCCGAGGAGACCAG
  GCAGCTGCTGGAACACAAATTTGACTATATCTTCTTCACAGGAAGTCCTCGGGTAGGCAAGATCGTCATG
  ACTGCTGCTGCCAAACACCTGACACCCATCACCCTGGAGCTGGGGGGTAAGAACCCCTGCTATGTGGATG
  ACAACTGCGACCCCCAGACAGTGGCCAACCGTGTGGCCTGGTTCCGCTATTTCAATGCCGGCCAGACCTG
  TGTGGCCCCCGATTATATCCTGTGTAGCCAGGAGATGCAGGAGCGGTTGGTGCCTGCCCTGCAGAATTCC
  ATCACGCGTTTCTATGGAGATAACCCACAGACCTCCCCCAACCTGGGCAGAATCATCAACCAGAAGCATT
  TCAAGAGGCTCCAGGGATTGCTGGGCTGTGGCCGTGTGGCCATCGGTGGCCAGAGTGATGAGGGAGAGCG
  CTACATTGCCCCCACAGTGCTGGTAGACGTGCAGGAGACAGAGCCCGTGATGCAGGAGGAGATCTTTGGG
  CCCATCCTGCCCCTGGTGACTGTGAGAAGCCTGGATGAAGCCATTGAGTTCATCAACCGGCGGGAGAAGC
  CTCTGGCGCTGTATGCCTTCTCCAACAACAACCAGGTGGTGAATCAGATGTTGGAACGCACCAGCAGCGG
  TGGCTTTGGAGGCAACGATGGCTTCCTCTACCTGACCCTACCAGCTCTGCCTTTGGGGGGAGTTGGCAAC
  AGCGGGATGGGCAGGTACCATGGCAAGTTCTCCTTTGACACCTTCTCCCACCATCGTGCCTGCCTGCTGC
  GCAGCCCTGGGATGGAAAAGCTTAATGACCTCCGTTACCCGCCTTATGGTCCCTGGAACCAACAACTGAT
  AAGCTGGGCCATAGGCTCCCGGAGCTGCACCCTCCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224974 representing NM_001177438
  Red=Cloning site Green=Tags(s)
  
  MSAAETGSEPSQGAGPSEATLHSLREAFNAGRTRPTEFRTAQLRSLGRFLQENKELLQDALAKDVGKSGF
  ESDMSEIILCENEVDLALKNLQTWMKDEPVSTNLLTKLSSAFIRKEPFGLVLIIAPWNYPVNLMIIPLVG
  AIAAGNCVVLKPSEISKNTEKVLAELLPQYLDQSCFAVMLGGPEETRQLLEHKFDYIFFTGSPRVGKIVM
  TAAAKHLTPITLELGGKNPCYVDDNCDPQTVANRVAWFRYFNAGQTCVAPDYILCSQEMQERLVPALQNS
  ITRFYGDNPQTSPNLGRIINQKHFKRLQGLLGCGRVAIGGQSDEGERYIAPTVLVDVQETEPVMQEEIFG
  PILPLVTVRSLDEAIEFINRREKPLALYAFSNNNQVVNQMLERTSSGGFGGNDGFLYLTLPALPLGGVGN
  SGMGRYHGKFSFDTFSHHRACLLRSPGMEKLNDLRYPPYGPWNQQLISWAIGSRSCTLL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177438
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177438.1, NP_001170909.1
• RefSeq Size: 2161 bp
• RefSeq ORF: 1440 bp
• Locus ID: 621603
• UniProt ID: E9Q3E1
• Cytogenetics: 19 A
• Gene Summary: Oxidizes medium and long chain aldehydes into non-toxic fatty acids.[UniProtKB/Swiss-Prot Function]