Lrp6 (NM_008514) Mouse Tagged ORF Clone

CAT#: MG212053

Lrp6 (tGFP-tagged) - Mouse low density lipoprotein receptor-related protein 6 (Lrp6)

Product Images

Circular map for MG212053

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lrp6
• Synonyms: C030016K15Rik; Cd; Gw; ska26; ska; skax26
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212053 representing NM_008514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCCGTGCTGAGGAGCCTCCTGGCCTGCAGCTTCTGCGTGCTGCTGAGAGCGGCCCCTTTGTTGC
  TTTATGCAAACAGACGGGACTTGAGATTGGTTGATGCTACAAATGGCAAAGAGAATGCAACGATTGTAGT
  TGGAGGCTTGGAGGATGCAGCTGCGGTGGACTTTGTGTTTGGTCATGGCTTGATATACTGGAGTGATGTC
  AGCGAAGAAGCCATTAAACGAACAGAATTTAACAAAACTGAAAGTGTACAGAATGTTGTTGTTTCTGGAT
  TATTGTCCCCGGATGGGCTGGCATGTGATTGGCTTGGAGAAAAATTGTACTGGACAGATTCTGAAACTAA
  TCGTATTGAAGTTTCTAATTTAGATGGATCTTTACGAAAAGTTTTATTTTGGCAAGAGTTGGATCAACCC
  AGAGCTATTGCCTTAGATCCATCAAGTGGGTTCATGTACTGGACAGACTGGGGAGAAGTGCCAAAGATAG
  AACGTGCTGGGATGGATGGCTCAAGTCGCTTCGTTATAATAAACACAGAGATTTACTGGCCAAACGGACT
  GACTCTGGACTATCAGGAGCGGAAGCTTTACTGGGCCGATGCAAAACTTAATTTCATCCATAAATCAAAC
  CTGGATGGAACAAACCGGCAGGCAGTGGTTAAAGGTTCCCTTCCACATCCTTTTGCCTTGACGTTATTTG
  AGGACACATTGTACTGGACTGACTGGAATACACACTCTATTTTGGCTTGCAACAAATATACTGGCGAGGG
  TCTGCGTGAAATTCATTCTAACATCTTCTCTCCCATGGATATACATGCTTTCAGCCAACAGAGGCAGCCA
  AATGCTACAAATCCATGTGGAATTGATAATGGTGGTTGTTCCCATTTGTGTTTGATGTCTCCAGTCAAGC
  CTTTTTATCAGTGTGCTTGCCCAACTGGGGTCAAGCTGCTGGAGAATGGAAAGACCTGCAAAGATGGTGC
  CACTGAACTATTGCTGTTAGCCCGACGGACAGACTTGAGGCGAATTTCTTTGGATACACCCGATTTTACT
  GACATTGTTCTGCAGTTAGAAGATATCCGGCATGCCATTGCCATAGACTATGACCCTGTAGAAGGCTACA
  TATACTGGACGGATGACGAAGTGAGGGCTATCCGTCGCTCCTTCATAGATGGATCTGGCAGTCAGTTTGT
  GGTCACGGCCCAGATTGCTCATCCTGATGGTATTGCTGTTGACTGGGTTGCAAGGAACCTGTACTGGACA
  GACACTGGCACGGATCGTATAGAAGTGACAAGGCTCAATGGGACCATGAGGAAGATCTTGATTTCAGAGG
  ACTTAGAGGAGCCCCGGGCTATCGTGTTAGATCCCATGGTTGGGTACATGTATTGGACAGACTGGGGAGA
  AATCCCAAAAATAGAGCGAGCTGCTCTGGACGGATCTGACCGAGTAGTTCTTGTCAACACTTCCCTTGGT
  TGGCCAAACGGCTTAGCCCTGGATTATGATGAAGGCACAATATACTGGGGAGATGCCAAAACAGACAAAA
  TTGAGGTTATGAATACCGATGGCACCGGGAGGCGAGTGCTGGTGGAAGACAAGATCCCTCACATATTTGG
  GTTTACCTTGCTGGGTGACTATGTTTACTGGACTGACTGGCAGAGGCGGAGCATCGAGAGAGTACACAAA
  CGGAGCGCAGAGAGGGAGGTCATCATAGATCAGCTGCCAGACCTCATGGGACTGAAGGCCACAAGTGTTC
  ACAGAATCATTGGTTCTAACCCCTGTGCTGAGGACAATGGAGGATGTAGCCATCTTTGCCTGTACAGGCC
  TCAGGGGCTTCGATGCGCCTGTCCCATTGGCTTTGAGCTCATCAGTGACATGAAGACATGCATTGTCCCC
  GAGGCTTTCCTTCTGTTCTCGAGGAGAGCGGATATCAGACGCATATCTTTGGAAACAAACAACAACAATG
  TGGCCATTCCTCTCACTGGTGTCAAAGAAGCCTCTGCTTTGGATTTTGATGTCACAGACAACAGGATTTA
  CTGGACTGATATATCACTGAAGACTATTAGCAGAGCCTTTATGAATGGCAGTGCACTGGAACATGTGGTA
  GAGTTTGGCTTAGATTATCCAGAAGGCATGGCAGTGGACTGGCTTGGGAAGAACTTATACTGGGCAGACA
  CAGGAACAAATCGCATTGAGGTATCGAAGTTGGACGGACAGCACCGACAGGTTTTGGTATGGAAAGACCT
  TGACAGTCCTCGAGCTCTGGCACTGGATCCTGCTGAAGGGTTTATGTATTGGACTGAGTGGGGAGGCAAG
  CCTAAGATTGACAGGGCTGCTATGGATGGAAGTGAACGCACTACATTAGTTCCAAATGTAGGCCGAGCAA
  ATGGTCTCACCATCGACTATGCTAAAAGGCGGCTTTACTGGACAGACCTGGACACTAACCTAATAGAATC
  CTCAGATATGCTCGGACTCAACCGTGAAGTTATAGCAGATGACTTGCCTCATCCTTTTGGCTTAACTCAG
  TACCAAGATTACATCTACTGGACAGACTGGAGCCGACGCAGCATTGAACGTGCCAACAAAACCAGTGGCC
  AAAACCGCACCATCATCCAGGGCCATTTGGACTATGTGATGGACATCCTGGTCTTCCACTCTTCCCGGCA
  GGCAGGGTGGAATGAGTGTGCCTCCAGCAACGGGCACTGCTCCCACCTCTGCTTGGCTGTGCCCGTCGGA
  GGTTTTGTGTGTGGATGCCCTGCCCACTACTCCCTGAATGCTGACAACAGGACCTGCAGTGCTCCCACCA
  CCTTCCTGCTCTTCAGTCAGAAGAGCGCCATCAACCGCATGGTGATTGATGAACAACAGAGCCCTGACAT
  CATCCTTCCTATCCACAGCCTTCGGAACGTCCGGGCCATTGACTATGACCCTTTGGACAAGCAGCTCTAC
  TGGATTGACTCTCGACAAAACTCCATACGAAAGGCACATGAAGATGGTGGCCAGGGTTTTAATGTAGTTG
  CAAACTCAGTCGCAAATCAGAACCTTGAAATACAGCCCTATGATCTCAGCATTGATATTTATAGCCGTTA
  CATCTACTGGACCTGTGAAGCTACCAATGTCATTGATGTGACGAGATTAGATGGACGATCAGTTGGAGTG
  GTTCTAAAAGGCGAGCAAGACAGACCTCGAGCCATTGTGGTAAACCCCGAGAAAGGGTATATGTATTTTA
  CCAATCTTCAGGAAAGATCTCCTAAAATTGAACGGGCTGCATTGGATGGTACAGAACGAGAGGTCCTCTT
  TTTCAGTGGCTTAAGTAAACCAATTGCTTTGGCTCTTGATAGCAAGCTGGGCAAGCTCTTCTGGGCTGAC
  TCAGATCTCCGGCGAATTGAAAGCAGTGATCTCTCAGGTGCCAACAGGATCGTGCTAGAAGACTCTAATA
  TATTACAGCCTGTGGGCCTGACCGTGTTTGAAAACTGGCTCTATTGGATTGATAAACAGCAGCAGATGAT
  TGAAAAAATTGACATGACTGGTCGAGAAGGAAGAACCAAGGTCCAGGCTCGAATTGCTCAGCTGAGTGAC
  ATCCATGCAGTAAAGGAGCTGAACCTTCAGGAGTACAGACAGCACCCTTGTGCCCAGGATAATGGTGGCT
  GTTCACATATCTGCCTTGTAAAAGGAGATGGTACGACAAGATGCTCCTGCCCCATGCACTTAGTTCTGCT
  TCAGGATGAGCTGTCCTGTGGAGAGCCTCCAACGTGTTCTCCTCAGCAGTTTACCTGCTTCACTGGGGAC
  ATTGACTGCATCCCTGTGGCTTGGCGGTGTGATGGGTTCACTGAGTGCGAAGACCACAGCGATGAACTCA
  ATTGTCCCGTGTGCTCAGAGTCTCAGTTCCAGTGTGCCAGCGGGCAGTGCATTGATGGTGCCCTTCGATG
  CAATGGCGATGCGAACTGCCAGGACAAATCAGATGAGAAGAACTGTGAAGTGCTTTGTTTAATTGATCAG
  TTCCGCTGTGCCAATGGTCAGTGCGTTGGAAAGCACAAGAAATGTGACCACAGTGTGGACTGCAGTGACA
  GATCTGACGAGCTGGACTGTTATCCAACTGAGGAGCCAGCACCACAAGCCACCAACACAGTTGGTTCTGT
  TATTGGAGTAATTGTCACCATTTTTGTGTCTGGAACCATATACTTTATCTGCCAGAGGATGCTGTGTCCT
  CGTATGAAGGGAGACGGGGAGACCATGACTAACGACTATGTGGTTCACAGCCCGGCGTCTGTGCCCCTTG
  GTTATGTTCCTCACCCAAGCTCTCTCTCTGGATCTCTTCCAGGAATGTCTCGAGGCAAATCAATGATCAG
  TTCCCTCAGTATCATGGGGGGAAGCAGTGGGCCCCCCTATGATCGAGCGCACGTCACGGGAGCCTCCTCA
  AGCAGTTCTTCCAGTACCAAAGGCACTTACTTCCCTGCAATTTTGAACCCACCACCATCGCCTGCCACAG
  AAAGATCCCATTATACCATGGAATTTGGTTATTCTTCCAACAGTCCTTCCACACATAGGTCCTACAGCTA
  TAGGCCGTACAGCTACCGGCACTTTGCACCGCCCACCACACCCTGCAGCACTGATGTCTGTGACAGTGAC
  TATGCTCCTAGCCGGAGGATGACCTCGGTGGCAACAGCCAAGGGCTACACCAGTGACGTGAACTATGACT
  CAGAACCTGTGCCCCCACCGCCCACACCCCGAAGCCAGTACTTGTCAGCGGAGGAGAACTATGAAAGCTG
  CCCCCCTTCCCCATACACGGAGAGGAGTTACTCCCACCACCTCTACCCGCCACCACCCTCCCCCTGCACG
  GACTCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212053 representing NM_008514
  Red=Cloning site Green=Tags(s)
  
  MGAVLRSLLACSFCVLLRAAPLLLYANRRDLRLVDATNGKENATIVVGGLEDAAAVDFVFGHGLIYWSDV
  SEEAIKRTEFNKTESVQNVVVSGLLSPDGLACDWLGEKLYWTDSETNRIEVSNLDGSLRKVLFWQELDQP
  RAIALDPSSGFMYWTDWGEVPKIERAGMDGSSRFVIINTEIYWPNGLTLDYQERKLYWADAKLNFIHKSN
  LDGTNRQAVVKGSLPHPFALTLFEDTLYWTDWNTHSILACNKYTGEGLREIHSNIFSPMDIHAFSQQRQP
  NATNPCGIDNGGCSHLCLMSPVKPFYQCACPTGVKLLENGKTCKDGATELLLLARRTDLRRISLDTPDFT
  DIVLQLEDIRHAIAIDYDPVEGYIYWTDDEVRAIRRSFIDGSGSQFVVTAQIAHPDGIAVDWVARNLYWT
  DTGTDRIEVTRLNGTMRKILISEDLEEPRAIVLDPMVGYMYWTDWGEIPKIERAALDGSDRVVLVNTSLG
  WPNGLALDYDEGTIYWGDAKTDKIEVMNTDGTGRRVLVEDKIPHIFGFTLLGDYVYWTDWQRRSIERVHK
  RSAEREVIIDQLPDLMGLKATSVHRIIGSNPCAEDNGGCSHLCLYRPQGLRCACPIGFELISDMKTCIVP
  EAFLLFSRRADIRRISLETNNNNVAIPLTGVKEASALDFDVTDNRIYWTDISLKTISRAFMNGSALEHVV
  EFGLDYPEGMAVDWLGKNLYWADTGTNRIEVSKLDGQHRQVLVWKDLDSPRALALDPAEGFMYWTEWGGK
  PKIDRAAMDGSERTTLVPNVGRANGLTIDYAKRRLYWTDLDTNLIESSDMLGLNREVIADDLPHPFGLTQ
  YQDYIYWTDWSRRSIERANKTSGQNRTIIQGHLDYVMDILVFHSSRQAGWNECASSNGHCSHLCLAVPVG
  GFVCGCPAHYSLNADNRTCSAPTTFLLFSQKSAINRMVIDEQQSPDIILPIHSLRNVRAIDYDPLDKQLY
  WIDSRQNSIRKAHEDGGQGFNVVANSVANQNLEIQPYDLSIDIYSRYIYWTCEATNVIDVTRLDGRSVGV
  VLKGEQDRPRAIVVNPEKGYMYFTNLQERSPKIERAALDGTEREVLFFSGLSKPIALALDSKLGKLFWAD
  SDLRRIESSDLSGANRIVLEDSNILQPVGLTVFENWLYWIDKQQQMIEKIDMTGREGRTKVQARIAQLSD
  IHAVKELNLQEYRQHPCAQDNGGCSHICLVKGDGTTRCSCPMHLVLLQDELSCGEPPTCSPQQFTCFTGD
  IDCIPVAWRCDGFTECEDHSDELNCPVCSESQFQCASGQCIDGALRCNGDANCQDKSDEKNCEVLCLIDQ
  FRCANGQCVGKHKKCDHSVDCSDRSDELDCYPTEEPAPQATNTVGSVIGVIVTIFVSGTIYFICQRMLCP
  RMKGDGETMTNDYVVHSPASVPLGYVPHPSSLSGSLPGMSRGKSMISSLSIMGGSSGPPYDRAHVTGASS
  SSSSSTKGTYFPAILNPPPSPATERSHYTMEFGYSSNSPSTHRSYSYRPYSYRHFAPPTTPCSTDVCDSD
  YAPSRRMTSVATAKGYTSDVNYDSEPVPPPPTPRSQYLSAEENYESCPPSPYTERSYSHHLYPPPPSPCT
  DSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008514
• ORF Size: 4839 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008514.4, NP_032540.2
• RefSeq Size: 9368 bp
• RefSeq ORF: 4842 bp
• Locus ID: 16974
• UniProt ID: O88572
• Cytogenetics: 6 65.37 cM
• Gene Summary: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).[UniProtKB/Swiss-Prot Function]