Slc26a1 (NM_174870) Mouse Tagged ORF Clone

CAT#: MG210148

Slc26a1 (tGFP-tagged) - Mouse solute carrier family 26 (sulfate transporter), member 1 (Slc26a1)

Product Images

Circular map for MG210148

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc26a1
• Synonyms: Sat1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210148 representing NM_174870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCTTCTCCTGAGCCCCAACAGAAGGGTGGGACACTGGTACTGGTCCGACGGCAGCCTCCTGTGT
  CCCAGGGCTTGCTGGAAACACTGAAGGCCAGGCTGAAGAAGAGCTGCACCTGCAGTATGCCATGTGCTCA
  GGCTCTGGTGCAGGGTCTGTTTCCTGCCATACACTGGCTGCCCCAGTACCGCCTTAAGGAATACCTAGCA
  GGTGATGTCATGTCAGGATTGGTCATTGGCATTATCCTGGTGCCACAGGCCATAGCCTACTCACTGCTGG
  CTGGGTTACAACCCATCTATAGCCTCTACACTTCCTTCTTTGCCAACCTGATCTACTTCCTCATGGGCAC
  CTCCCGCCATGTTAATGTGGGCATCTTCAGCCTGCTGTGTCTCATGGTGGGTCAGGTGGTGGACAGAGAA
  CTCCAGTTGGCTGGCTTTGACCCTTCCCAGGATTCTCTAGGGCCCAAGAACAATGACAGCACCCTCAACA
  ACTCAGCTACAACACTGATCATTGGGCTACAGGACTGCAGACGGGACTGCTATGCCATTCGGGTTGCCAC
  TGCCCTTACTCTGATGGCCGGGCTTTATCAGGTCCTCATGGGTATCCTCCGGCTGGGCTTCGTGTCTACC
  TATCTCTCACAACCACTGCTCGATGGCTTTGCTATGGGAGCTTCTGTGACCATCTTGACTTCTCAGGCAA
  AACACATGCTGGGTGTGCAGATCCCTCGGCACCAGGGCCTAGGCATGGTGGTCCACACATGGTTGAGCTT
  GCTGCAGAATGTGGGACAGGCCAACATATGTGATGTGGTCACCAGTGCCCTGTGCCTAGGAGTGCTGCTG
  GCAGCTAAGGAACTCTCAGATCGCTACCGACACCGTCTGAAGGTGCCAATACCCACAGAGTTATTTGTTA
  TTGTGGTGGCCACAATTGTGTCCCATTTTGGACAGCTCCATACACGGTTTGACTCGAGGGTGGCTGGCAA
  CATTCCCACTGGTTTTGTGGCCCCACAGGTACCAGACCCTAAGATAATGTGGCGTGTGGCCCTGGATGCC
  ATGTCCCTGGCCCTCGTGGGCTCAGCCTTCTCCATCTCCTTGGCAGAAATGTTTGCCCGTAGTCATGGCT
  ACTCTGTCAGTGCCAACCAAGAGCTGCTAGCTGTGGGCTGCTGCAATGTGCTGCCTGCCTTCTTCCACTG
  TTTTGCCACTAGTGCTGCTCTGTCCAAAACTCTGGTGAAGATAGCCACTGGCTGCCAGACCCAGTTGTCC
  AGTGTGGTCAGTGCCGCTGTGGTGTTGCTGGTGCTGCTGGTGCTGGCTCCACTGTTTCATGATCTGCAGC
  GATGTGTGCTAGCTTGTATCATTGTTGTCAGCCTGCGGGGGGCGCTGCGCAAGGTGAAGGATCTCCCACA
  ACTTTGGCGGCTAAGCCCTGCCGATGCACTGGTCTGGGTGGCTACTGCAGCTACCTGTGTGCTAGTCAGC
  ACCGAGGCTGGACTATTAGCTGGAGTGTTCTTCTCCCTGCTTAGCCTGGCAGGCCGTACACAGCGTCCAC
  GGGCTGCCCTTCTTGCCCGAATTGGAGACTCCACCTTCTATGAGGATGCTGCTGAATTTGAGGGCCTCCT
  GCCCCCACCTGAGGTGCGAGTGTTCCGTTTCACAGGTCCACTTTACTACGCCAACAAGGACTTCTTCCTT
  CGGTCTCTCTACAGACTCACAGGGTTGGATGCAGGGCACTCAGCCACCAGGAAGGATCAGGGCCCGGAGG
  TGGGTGTCAGTAACAGAAGTCTTGTTGATGGCAAGGATCTGGGTTCAGTGAGCAGTGGGGCTGGGCTGGT
  GGTACCCCTGGCATTTGGTTTCCACACAGTGGTCATTGACTGTGCACCACTGCTGTTCCTGGATGTGGCT
  GGCATGGCCACATTGAAAGACCTCCGCAGAGACTACAGGGCCTTGGACATCACCCTGCTTCTGGCTTGCT
  GCAGTCCCTCGGTGAGAGACACGTTGAGAAAAGGGGGCTTCCTTGGGGAAGAACAGGGAGCTGAAAATGA
  GCTGCTATTCCCCAGTGTGCACAGTGCTGTGGAGGCTGCATGTGCCCGCCGTGAGGAGCTGCTGGCTGCT
  GACTCTGCCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210148 representing NM_174870
  Red=Cloning site Green=Tags(s)
  
  MDASPEPQQKGGTLVLVRRQPPVSQGLLETLKARLKKSCTCSMPCAQALVQGLFPAIHWLPQYRLKEYLA
  GDVMSGLVIGIILVPQAIAYSLLAGLQPIYSLYTSFFANLIYFLMGTSRHVNVGIFSLLCLMVGQVVDRE
  LQLAGFDPSQDSLGPKNNDSTLNNSATTLIIGLQDCRRDCYAIRVATALTLMAGLYQVLMGILRLGFVST
  YLSQPLLDGFAMGASVTILTSQAKHMLGVQIPRHQGLGMVVHTWLSLLQNVGQANICDVVTSALCLGVLL
  AAKELSDRYRHRLKVPIPTELFVIVVATIVSHFGQLHTRFDSRVAGNIPTGFVAPQVPDPKIMWRVALDA
  MSLALVGSAFSISLAEMFARSHGYSVSANQELLAVGCCNVLPAFFHCFATSAALSKTLVKIATGCQTQLS
  SVVSAAVVLLVLLVLAPLFHDLQRCVLACIIVVSLRGALRKVKDLPQLWRLSPADALVWVATAATCVLVS
  TEAGLLAGVFFSLLSLAGRTQRPRAALLARIGDSTFYEDAAEFEGLLPPPEVRVFRFTGPLYYANKDFFL
  RSLYRLTGLDAGHSATRKDQGPEVGVSNRSLVDGKDLGSVSSGAGLVVPLAFGFHTVVIDCAPLLFLDVA
  GMATLKDLRRDYRALDITLLLACCSPSVRDTLRKGGFLGEEQGAENELLFPSVHSAVEAACARREELLAA
  DSAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174870
• ORF Size: 2112 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174870.2, NP_777359.2
• RefSeq Size: 3821 bp
• RefSeq ORF: 2115 bp
• Locus ID: 231583
• UniProt ID: P58735
• Cytogenetics: 5 F
• Gene Summary: Mediates sulfate transport with high affinity (PubMed:12217875, PubMed:27210743). Mediates oxalate transport (PubMed:12217875). Mediates bicarbonate transport (PubMed:27210743). Does not accept succinate as cosubstrate (By similarity).[UniProtKB/Swiss-Prot Function]