Lancl1 (NM_021295) Mouse Tagged ORF Clone

CAT#: MG206265

Lancl1 (tGFP-tagged) - Mouse LanC (bacterial lantibiotic synthetase component C)-like 1 (Lancl1)

Product Images

Circular map for MG206265

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lancl1
• Synonyms: AW124738; Gpr69; Gpr69a; p40
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206265 representing NM_021295
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGAGGGCCTTTCCGAATCCTTATGCCGATTATAACAAGTCGCTGGCCGAAAACTACTTTGATT
  CTACTGGGAGGCTGACTCCTGAGTTCTCACATCGCTTGACCAATAAGATCCGAGAGCTCCTTCAGCAAAT
  GGAGAGAGGCCTAAAGTCTGCAGACCCTCGGGATGGCACTGGGTACACTGGCTGGGCAGGCATCGCTGTG
  CTTTACCTCCACCTCCACAATGTGTTTGGGGACCCAGCCTATCTACAGATGGCGCACAGCTATGTAAAGC
  AAAGCCTCAACTGTCTGAGCAGGCGTTCCATCACCTTCCTGTGTGGCGATGCGGGCCCCCTGGCTGTGGC
  TGCTGTGCTGTACCACAAGATGAACAGTGAGAAGCAGGCCGAGGAGTGCATCACACGGCTCATTCACCTA
  AATAAGATTGATCCCCACGTACCAAATGAAATGCTCTATGGCCGCATAGGCTACATCTTTGCTCTGCTTT
  TTGTCAATAAGAACTTTGGAGAGGAGAAGATTCCTCAGAGCCATATTCAGCAGATTTGTGAAAACATCTT
  AACCTCTGGGGAAAACCTATCTAGAAAGAGAAACTTGGCGGCAAAGTCTCCACTGATGTATGAGTGGTAC
  CAGGAATATTACGTGGGGGCTGCTCATGGCTTGGCAGGCATTTATTACTACCTGATGCAGCCCAGCCTTC
  AGGTGAACCAAGGAAAGTTGCATAGTTTGGTGAAGCCCAGTGTAGACTTTGTCTGCCGGCTGAAGTTTCC
  TTCCGGCAATTACCCTCCATGTTTGGATGATACCAGAGACCTGCTTGTCCATTGGTGCCACGGTGCTCCT
  GGGGTCATCTATATGCTCATCCAAGCATACAAGGTGTTCAAAGAAGAGCGTTACCTGTGTGATGCCCAGC
  AGTGTGCTGACGTGATCTGGCAGTACGGGCTACTGAAGAAGGGCTACGGGCTGTGCCATGGTGCTGCAGG
  GAATGCCTACGCTTTCCTGGCACTCTACAACCTCACACAGGATCTGAAGTATCTGTACAGGGCCTGCAAG
  TTTGCTGAGTGGTGCTTAGACTACGGGGAGCACGGATGCAGGACAGCGGACACCCCCTTCTCCCTCTTTG
  AAGGGATGGCTGGAACAATATATTTCTTGGCTGACCTGTTAGTCCCCACAAAGGCCAAGTTCCCTGCATT
  TGAACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206265 representing NM_021295
  Red=Cloning site Green=Tags(s)
  
  MAQRAFPNPYADYNKSLAENYFDSTGRLTPEFSHRLTNKIRELLQQMERGLKSADPRDGTGYTGWAGIAV
  LYLHLHNVFGDPAYLQMAHSYVKQSLNCLSRRSITFLCGDAGPLAVAAVLYHKMNSEKQAEECITRLIHL
  NKIDPHVPNEMLYGRIGYIFALLFVNKNFGEEKIPQSHIQQICENILTSGENLSRKRNLAAKSPLMYEWY
  QEYYVGAAHGLAGIYYYLMQPSLQVNQGKLHSLVKPSVDFVCRLKFPSGNYPPCLDDTRDLLVHWCHGAP
  GVIYMLIQAYKVFKEERYLCDAQQCADVIWQYGLLKKGYGLCHGAAGNAYAFLALYNLTQDLKYLYRACK
  FAEWCLDYGEHGCRTADTPFSLFEGMAGTIYFLADLLVPTKAKFPAFEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021295
• ORF Size: 4274 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021295.3, NP_067270.1
• RefSeq Size: 4270 bp
• RefSeq ORF: 1200 bp
• Locus ID: 14768
• UniProt ID: O89112
• Cytogenetics: 1 C3
• Gene Summary: Functions as glutathione transferase (PubMed:25158856). Catalyzes conjugation of the glutathione (GSH) to artificial substrates 1-chloro-2,4-dinitrobenzene (CDNB) and p-nitrophenyl acetate (PubMed:25158856). Mitigates neuronal oxidative stress during normal postnatal development and in response to oxidative stresses probably through GSH antioxidant defense mechanism (PubMed:25158856). May play a role in EPS8 signaling. Binds glutathione (By similarity).[UniProtKB/Swiss-Prot Function]