Crisp3 (NM_009639) Mouse Tagged ORF Clone

CAT#: MG202984

Crisp3 (tGFP-tagged) - Mouse cysteine-rich secretory protein 3 (Crisp3)

Product Images

Circular map for MG202984

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Crisp3
• Synonyms: Aeg2; CRISP-3; CRS3; SGP28
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202984 representing NM_009639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTAATGCTTGTGCTGTTCTTCCTGGCTGCTGTACTGCCCCCATCCCTTCTTCAAGATAACTCTC
  AGGAGAACAGTCTTGAGAAACTTTCAACCAGTAAAAAATCAGTCCAAGAAGAGATTGTAAGCAAGCACAA
  CCAATTGAGACGAAAGGTTTCTCCATCTGGCAGTGACTTACTAAATATGGAATGGAACTATGATGCTCAA
  GTGAATGCTCAGCAACGGGCAGACAAGTGTACATTCAGTCACAGTCCTATAGAACTCAGGACAACTAATT
  TAAAATGTGGTGAGAATTTGTTCATGTCATCTTACCTTGTACCATGGTCTTCTGTAATCCAAGGATGGTA
  TAATGAATCCAAAGGTCTTATATTTGGTGTGGGCCCAAAGCAAAATGTTAGTGTGGTTGGACATCATACT
  CAGGTTGTTTGGAAATCAAATTTACAAGTTGCATGTGGAGTTGCTGAATGCCCTGAAAATCCACTGAGAT
  ACTTTTATGTTTGTCGCTATTGTCCTGTATTGAATTACAGTGGCCATTATCCAAGCAGGCCATACCTAGC
  TTACACAGCAAGAGCACCATGTGCCAGTTGTCCTGATCGCTGTGAAGATGGACTGTGCACCAAGAGTTGT
  CAATATAAGGATATGTCTTTTTGGTGTAAACGTCTGGAATACGTCTGTAAACATCCAGGTCTTAAAAAAC
  GTTGCCTAGCTACATGCCAATGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202984 representing NM_009639
  Red=Cloning site Green=Tags(s)
  
  MALMLVLFFLAAVLPPSLLQDNSQENSLEKLSTSKKSVQEEIVSKHNQLRRKVSPSGSDLLNMEWNYDAQ
  VNAQQRADKCTFSHSPIELRTTNLKCGENLFMSSYLVPWSSVIQGWYNESKGLIFGVGPKQNVSVVGHHT
  QVVWKSNLQVACGVAECPENPLRYFYVCRYCPVLNYSGHYPSRPYLAYTARAPCASCPDRCEDGLCTKSC
  QYKDMSFWCKRLEYVCKHPGLKKRCLATCQC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009639
• ORF Size: 723 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009639.3
• RefSeq Size: 1406 bp
• RefSeq ORF: 726 bp
• Locus ID: 11572
• UniProt ID: Q03402
• Cytogenetics: 17 19.45 cM
• Gene Summary: This protein is supposed to help spermatozoa undergo functional maturation while they move from the testis to the ductus deferens.[UniProtKB/Swiss-Prot Function]