Tlr1 (NM_001276445) Mouse Untagged Clone

CAT#: MC228948

Tlr1 (untagged) - Mouse toll-like receptor 1 (Tlr1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tlr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228948 representing NM_001276445
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAAACCAAATTCCCTCATCTTCTACTGTATCATTGTTTTAGGACTGACACTTATGAAAATCCAAT
  TATCTGAGGAATGTGAGCTTATCATAAAGAGGCCAAACGCAAACCTTACCAGAGTGCCCAAGGACCTACC
  CTTGCAAACAACTACTTTAGATCTATCACAAAACAATATATCTGAGCTTCAGACTTCTGACATCCTCTCA
  TTGTCCAAGCTGAGGGTCCTGATAATGTCCTACAACAGACTCCAGTATCTTAATATCAGTGTTTTCAAAT
  TCAACACAGAGCTGGAATATTTGGATTTGTCCCACAATGAGCTAAAGGTGATCTTGTGCCACCCAACAGT
  CAGCCTCAAGCATTTGGACCTCTCCTTTAATGCCTTTGATGCCCTGCCTATATGCAAAGAATTTGGCAAC
  ATGTCCCAACTACAGTTCCTGGGGTTGAGCGGTTCTCGGGTACAAAGTTCAAGTGTGCAGCTGATTGCTC
  ATTTGAACATCAGTAAGGTTTTGCTGGTGTTAGGAGATGCTTATGGGGAAAAAGAAGACCCCGAATCTCT
  TCGGCACGTTAGCACTGAGACTCTGCATATTGTTTTCCCGTCGAAAAGAGAATTCCGTTTTCTTCTGGAT
  GTGTCCGTCAGCACTACGATCGGTTTGGAACTGTCTAACATCAAGTGTGTGCTTGAAGACCAGGGCTGCT
  CTTATTTCTTACGTGCTTTGTCAAAGCTTGGAAAGAATCTGAAGCTCTCAAATCTTACCCTGAACAATGT
  GGAAACAACGTGGAATTCCTTCATTAATATCCTCCAGATAGTTTGGCATACGCCAGTCAAATATTTCTCA
  ATTTCAAATGTGAAGCTACAAGGTCAACTTGCCTTCAGGATGTTCAATTATTCTGACACTTCTCTGAAGG
  CTTTGTCGATACATCAAGTTGTCACTGATGTCTTCAGCTTCCCCCAAAGTTACATATACAGTATCTTTGC
  CAATATGAACATCCAAAACTTTACAATGTCTGGAACACACATGGTCCACATGCTGTGCCCGTCCCAAGTT
  AGCCCATTTCTGCATGTGGACTTTACAGATAACCTTTTAACAGACATGGTTTTTAAAGACTGTAGAAACT
  TAGTTAGATTGAAAACACTTAGTTTACAAAAGAATCAGTTAAAAAACCTTGAGAATATAATCCTCACATC
  TGCAAAGATGACATCCCTACAAAAACTAGACATTAGCCAGAATTCTCTAAGGTACAGCGATGGGGGAATC
  CCATGCGCCTGGACCCAGAGTTTGTTAGTTTTAAATTTGTCTTCGAATATGCTTACAGGCTCTGTCTTCA
  GATGCTTACCTCCCAAAGTCAAGGTCCTTGACCTTCACAACAACAGGATAATGAGCATCCCTAAAGATGT
  CACCCACCTGCAGGCTTTGCAGGAACTCAATGTAGCATCCAACTCCTTAACTGACCTTCCTGGGTGCGGG
  GCCTTCAGCAGCCTTTCTGTGCTGGTCATCGACCATAACTCAGTTTCCCATCCCTCTGAGGATTTCTTCC
  AGAGCTGTCAGAATATTAGATCCCTAACAGCGGGAAACAACCCATTCCAATGCACATGTGAGCTGAGGGA
  CTTTGTCAAGAACATAGGCTGGGTAGCAAGAGAAGTGGTGGAGGGCTGGCCTGACTCTTACAGGTGTGAC
  TACCCAGAAAGCTCTAGGGGAACTGCACTGAGGGACTTCCACATGTCTCCACTATCCTGTGATACTGTTC
  TGCTGACTGTCACCATCGGGGCCACTATGCTGGTGCTGGCTGTCACTGGGGCTTTCCTCTGTCTCTACTT
  TGACCTGCCCTGGTATGTGAGGATGCTGTGTCAGTGGACACAGACCAGGCACAGGGCCAGGCACATCCCC
  TTAGAGGAACTCCAGAGAAACCTCCAGTTCCATGCTTTTGTCTCATACAGTGGGCATGATTCTGCCTGGG
  TGAAGAACGAATTACTACCCAACCTAGAGAAAGATGACATCCAGATTTGCCTCCATGAGAGGAACTTTGT
  CCCTGGCAAGAGCATTGTGGAGAACATCATCAATTTCATTGAGAAGAGTTACAAGTCCATCTTTGTGCTG
  TCTCCCCACTTCATCCAGAGTGAGTGGTGTCATTATGAACTCTATTTTGCCCATCACAATCTCTTCCATG
  AAGGCTCTGATAACTTAATCCTCATCTTGCTGGCACCCATTCCCCAGTACTCCATCCCTACCAATTACCA
  CAAGCTCAAAACTCTCATGTCACGAAGGACCTATCTGGAATGGCCCACAGAGAAGAACAAGCATGGACTT
  TTTTGGGCAAACCTAAGAGCATCCATTAATGTTAAGCTGGTTAACCAGGCAGAAGGAACGTGTTACACAC
  AGCAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276445
• Insert Size: 2388 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276445.1, NP_001263374.1
• RefSeq Size: 3021 bp
• RefSeq ORF: 2388 bp
• Locus ID: 21897
• UniProt ID: Q9EPQ1
• Cytogenetics: 5 33.53 cM
• Gene Summary: Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). Acts as a coreceptor for M.tuberculosis lipoproteins LprG, LpqH and PhoS1 (pstS1), in conjunction with TLR2 and for some but not all lipoproteins CD14 and/or CD36. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.