Mmp8 (NM_008611) Mouse Untagged Clone

CAT#: MC216270

Mmp8 (untagged) - Mouse matrix metallopeptidase 8 (Mmp8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mmp8
• Synonyms: BB138268
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216270 representing NM_008611
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCGCCTGAAGACACTTCCATTACTGATCTTCCTCCACACACAGCTTGCCAATGCCTTCCCAGTAC
  CTGAACACCTGGAAGAGAAAAATATAAAAACTGCTGAGAATTACCTACGAAAATTCTACAACTTACCAAG
  CAATCAATTCCGGTCTTCGAGGAATGCCACGATGGTTGCAGAGAAGCTTAAAGAGATGCAGCGCTTCTTC
  AGCTTAGCAGAGACTGGGAAGTTAGATGCAGCTACAATGGGGATTATGGAAATGCCTCGATGTGGAGTGC
  CTGACTCTGGTGATTTCTTGCTAACCCCAGGAAGTCCCAAGTGGACACACACTAACCTGACCTACAGGAT
  TATAAACCATACGCCACAGCTGTCAAGGGCTGAAGTGAAAACAGCAATTGAGAAAGCTTTTCACGTCTGG
  AGTGTAGCATCGCCCCTGACCTTCACCGAGATCTTACAGGGAGAAGCAGACATCAACATTGCTTTCGTCT
  CAAGAGACCATGGTGACAATTCTCCATTTGATGGACCCAATGGAATCCTTGCCCATGCCTTTCAACCAGG
  CCAAGGTATTGGAGGAGATGCTCATTTTGATTCAGAAGAAACGTGGACTCAAGATTCCAAAAATTACAAC
  CTATTTCTCGTGGCTGCTCATGAATTTGGACATTCTTTGGGACTCTCTCACTCCACTGATCCTGGTGCCT
  TGATGTACCCAAACTATGCTTACAGGGAACCCAGCACCTATTCACTACCTCAAGATGATATCAATGGCAT
  TCAGACAATCTATGGACCTTCAGACAACCCCATCCAACCTACTGGGCCCAGCACACCCAAAGCCTGTGAC
  CCCCACCTGAGATTTGATGCTACTACCACACTCCGTGGGGAGATTTACTTCTTTAAAGACAAGTACTTCT
  GGAGACGGCATCCTCAGCTGAGAACAGTTGACCTCAATTTCATATCTCTGTTCTGGCCCTTCCTACCCAA
  CGGTCTTCAGGCTGCTTATGAAGATTTTGACAGAGACCTAGTTTTCCTATTTAAAGGCAGACAGTACTGG
  GCTCTAAGTGGCTATGACCTGCAGCAAGGTTACCCCAGAGATATATCCAACTATGGATTCCCAAGGAGTG
  TCCAAGCCATTGATGCAGCTGTTTCCTATAACGGGAAGACATACTTCTTCATAAACAACCAGTGCTGGAG
  ATATGACAATCAAAGAAGATCCATGGACCCAGGTTACCCCAAAAGCATACCAAGCATGTTCCCAGGAGTA
  AACTGTAGAGTCGATGCAGTTTTCCTGCAGGACTCCTTCTTCCTCTTCTTCAGTGGACCACAATATTTTG
  CATTTAATTTTGTCAGTCACAGAGTCACTAGAGTTGCAAGAAGCAATTTATGGCTTAACTGTTCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008611
• Insert Size: 1398 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008611.4, NP_032637.3
• RefSeq Size: 2453 bp
• RefSeq ORF: 1398 bp
• Locus ID: 17394
• UniProt ID: O70138
• Cytogenetics: 9 A1
• Gene Summary: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades types I, II and III collagens. Mice lacking the encoded protein exhibit abnormalities in the inflammatory responses to various agents. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Feb 2016]