Wdfy2 (NM_175546) Mouse Untagged Clone

CAT#: MC214269

Wdfy2 (untagged) - Mouse WD repeat and FYVE domain containing 2 (Wdfy2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wdfy2
• Synonyms: 5830485M08; B130024L21Rik; ProF; ZFYVE22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214269 representing NM_175546
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGAGATCCAGCCCCAGCCACTGACCCGCAAGCCGATCCTGCTGCAGCGGGTCGAGGGGTCGC
  AGGAGGTGGTCAACATGGCGGTGATTGTACCTAAAGAAGAGGGTGTCATCAGCGTCTCGGAGGACAGGAC
  AGTTCGTGTTTGGTTAAAGAGGGACAGTGGACAGTACTGGCCAAGCATCTACCATGCAATGCCTTCTCCA
  TGTTCATGCATGTCTTTTAACCCGGAAACAAGAAGACTGTCCATAGGTCTAGACAATGGTACAATCTCAG
  AGTTTATTTTGTCTGAAGATTATAACAAGATGACACCTGTGAAAAACTATCAAGCACATCAGAGCAGAGT
  AACGATGGTCCTGTTTGTTCTGGAACTGGAGTGGGTGCTGAGCACAGGACAGGACAAGCAGTTCGCCTGG
  CACTGCTCTGAGAGTGGACAGCGCCTGGGAGGTTACCGCACCAGTGCTGTGGCCTCAGGCCTGCAATTTG
  ATGTTGAAACCCGTCATGTGTTTATTGGCGATCACTCGGGCCAAGTAACCATCCTCAAACTGGAACAAGA
  AAACTGCACACTGCTCACATCCTTCAGAGGGCACACAGGAGGGGTGACAGCACTCTGCTGGGACCCGGTG
  CAGCGCGTGCTATTCTCAGGCAGTTCCGATCACTCTGTCATCATGTGGGACATCGGTGGGAGAAAAGGCA
  CGGCCATCGAACTCCAAGGACACAATGACAAAGTCCAGGCCCTCTCCTATGCACAGCACACACGACAGCT
  CATCTCATGTGGCGGTGACGGTGGCATTGTCGTCTGGAACATGGATGTGGAAAGGCAGGAGACCCCTGAG
  TGGTTGGACAGTGACTCCTGCCAAAAGTGTGACCAGCCTTTCTTCTGGAACTTCAAGCAAATGTGGGACA
  GTAAGAAAATTGGACTAAGACAGCACCACTGCCGGAAGTGTGGGAAGGCCGTGTGCGGCAAGTGTAGCTC
  CAAGCGCTCTTCCATCCCACTCATGGGCTTTGAGTTTGAAGTGCGGGTCTGCGACAGCTGCCACGAGGCC
  ATCACAGATGAAGAACGCGCCCCCACAGCCACCTTCCATGACAGTAAGCATAACATTGTGCATGTGCATT
  TCGATGCAACCAGGGGATGGTTACTGACTTCTGGAACTGACAAGGTAATTAAGCTGTGGGACATGACCCC
  AGTGGTGTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175546
• Insert Size: 1203 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175546.4, NP_780755.2
• RefSeq Size: 2011 bp
• RefSeq ORF: 1203 bp
• Locus ID: 268752
• UniProt ID: Q8BUB4
• Cytogenetics: 14 D1
• Gene Summary: Acts in an adapter protein-like fashion to mediate the interaction between the kinase PRKCZ and its substrate VAMP2 and increases the PRKCZ-dependent phosphorylation of VAMP2 (By similarity). Positively regulates adipocyte differentiation, by facilitating the phosphorylation and thus inactivation of the anti-adipogenetic transcription factor FOXO1 by the kinase AKT1 (PubMed:18388859). Plays a role in endosomal control of AKT2 signaling; required for insulin-stimulated AKT2 phosphorylation and glucose uptake and insulin-stimulated phosphorylation of AKT2 substrates (PubMed:20189988). Participates in transferrin receptor endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]