Human Furin ELISA Kit

CAT#: EA100575

Human Furin ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human Furin
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 312pg/ml-20,000pg/ml
• Sample Type: Cell culture supernates, tissue homogenates and Cell lysates.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of Human Furin
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human Furin antibody 1
  • Lyophilized recombinant human Furin standard 20ng/tubex2
  • Biotinylated anti- human Furin antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Furin(Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance
• Gene Symbol: FURIN
• Gene ID: 5045