Human PAI-1 ELISA Kit

CAT#: EA100546

Human PAI-1 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human PAI-1
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10,000pg/ml
• Sample Type: Cell culture supernates, Cell lysates, serum and plasma(heparin, EDTA, citrate ).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human PAI-1
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human PAI-1 antibody 1
  • Lyophilized recombinant human PAI-1 standard 10ng/tubex2
  • Biotinylated anti- human PAI-1 antibody 100ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 100ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing reagent 10ml
  • TMB stop solution 10ml
  • Wash Buffer (25x) 20 ml
  • Plate Sealers 4 Pieces
• Background: Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator(tPA) and urokinase(uPA), the activators of plasminogen and hence fibrinolysis(the physiological breakdown of blood clots). It is a serine protease inhibitor(serpin) protein(SERPINE1). PAI-1 is mainly produced by the endothelium(cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome(7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis(a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states(such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.
• Gene Symbol: SERPINE1
• Gene ID: 5054