Human MIP-1 alpha ELISA Kit

CAT#: EA100309

Human MIP-1 alpha ELISA Kit

Product Images

Representative standard curve for Human MIP-1 alpha PicoKine ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human MIP-1 alpha
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6pg/ml-1000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human MIP-1 alpha
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human MIP-1 alpha antibody 1
  • Lyophilized recombinant human MIP-1 alpha standard 10ng/tubex2
  • Biotinylated anti- human MIP-1 alpha antibody 100ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 100ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20 ml
• Background: Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5kDa.
• Gene Symbol: CCL3
• Gene ID: 6348
• Standard Curve:
  
  Representative standard curve for Human MIP-1 alpha PicoKine ELISA Kit