ROR1 Mouse Monoclonal Antibody [Clone ID: OTI3F8]

CAT#: TA806841

ROR1 mouse monoclonal antibody,clone OTI3F8

Product Images

IHC staining of FFPE human lymphoma tissue using anti-ROR1 mouse monoclonal antibody (TA806841) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human ROR1 plasmid (RC214967, hROR1), human ROR2 plasmid (RC215640, hROR2), mouse ROR1 plasmid (MR224803, mROR1),rat ROR1 plasmid (RR214709, rROR1), using anti-ROR1 antibody TA806841 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Specifications

Product Data

• Clone Name: OTI3F8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ROR1 (NP_005003) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 104.1 kDa
• Gene Name: receptor tyrosine kinase-like orphan receptor 1
• Database Link:
  NP_005003
  Entrez Gene 362550 RatEntrez Gene 4919 Human
  UniProt ID Q01973
• Background: This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012]
• Synonyms: dJ537F10.1; NTRKR1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane