Wilms Tumor Protein (WT1) Rabbit Monoclonal Antibody [Clone ID: OTIR5F11]

CAT#: TA591047

Rabbit monoclonal anti-WT1 Antibody, clone OTIR5F11

Product Images

Western blot analysis of extracts from 293-6E cell line (left lane) and 293-6E cells transfected with WT1 gene (right lane), using anti- WT1 rabbit monoclonal antibody (TA591047) at 1:500 dilution.

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  Immunohistochemical staining of paraffin-embedded human adjacent normal breast using rabbit monoclonal anti-WT1 clone OTIR5F11 monoclonal antibody @ 1:200 with detection kit Rabbit Polink1 (D13) HRP and DAB chromogen (TA591047; requires heat-induced epitope retrieval with Tris/ EDTA buffer (ACCEL), pH8.7 using BioCare Decloaker)

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  Immunohistochemical staining of paraffin-embedded human adjacent normal kidney using rabbit monoclonal anti-WT1 clone OTIR5F11 monoclonal antibody @ 1:200 with detection kit Rabbit Polink1 (D13) HRP and DAB chromogen (TA591047; requires heat-induced epitope retrieval with Tris/ EDTA buffer (ACCEL), pH8.7 using BioCare Decloaker)

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  Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using rabbit monoclonal anti-WT1 clone OTIR5F11 monoclonal antibody @ 1:100 with detection kit Rabbit Polink1 (D13) HRP and DAB chromogen (TA591047; requires heat-induced epitope retrieval with Tris/ EDTA buffer (ACCEL), pH8.7 using BioCare Decloaker)

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  Immunohistochemical staining of paraffin-embedded human testies using rabbit monoclonal anti-WT1 clone OTIR5F11 monoclonal antibody @ 1:200 with detection kit Rabbit Polink1 (D13) HRP and DAB chromogen (TA591047; requires heat-induced epitope retrieval with Tris/ EDTA buffer (ACCEL), pH8.7 using BioCare Decloaker)

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  Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using rabbit monoclonal anti-WT1 clone OTIR5F11 monoclonal antibody @ 1:100 with detection kit Rabbit Polink1 (D13) HRP and DAB chromogen (TA591047; requires heat-induced epitope retrieval with Tris/ EDTA buffer (ACCEL), pH8.7 using BioCare Decloaker)

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Specifications

Product Data

• Clone Name: OTIR5F11
• Applications: ELISA, IHC, WB
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human WT1 (NP_000369). The exact sequence is proprietary.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: Lot dependent; please refer to CoA along with shipment
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Predicted Protein Size: 56 kDa
• Gene Name: WT1 transcription factor
• Database Link:
  NP_000369
  Entrez Gene 22431 MouseEntrez Gene 24883 RatEntrez Gene 7490 Human
  UniProt ID P19544
• Synonyms: AWT1; GUD; NPHS4; WAGR; WIT-2; WT33

Reference Data

• Protein Families: Druggable Genome, Transcription Factors