IL17F Mouse Monoclonal Antibody [Clone ID: 4H1629.1]

CAT#: TA397218S

IL-17F Antibody

Product Images

Immunohistochemistry of Mouse Anti-IL-17F antibody. Tissue: human colon tissue. Fixation: formalin-fixed, paraffin-embedded. Primary antibody: isotype control (top left), Mouse Anti-IL-17F antibody (full right and bottom left) at 5 ug/ml.

Specifications

Product Data

• Clone Name: 4H1629.1
• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 0.5µg/mL
  IHC: 5µg/mL
  ELISA: 1:10,000 - 1:50,000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Anti-IL-17F (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17F produced in E.coli followed by hybridoma development.
• Specificity: Anti-Human IL-17F (MOUSE) Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17F protein. A BLAST analysis was used to suggest cross-reactivity with IL-17F from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17F from other sources has not been determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Stability: Expiration date is one (1) year from date of receipt.
• Gene Name: interleukin 17F
• Database Link:
  Entrez Gene 112744 Human
  UniProt ID Q96PD4
• Background: Anti-L-17F recognizes IL-17F (also known as Cytokine ML-1 or Interleukin-24). IL-17F is produced and secreted by CD8+ T cells, NK cells, NKT cells and LTi cells. The main functions of IL-17F are neutrophil recruitment and immunity to extracellular pathogen. More importantly, IL-17F drives inflammation and auto-immunity. IL-17A and IL-17F are by far the best characterized cytokines of the IL-17 cytokine family. IL-17F dimerizes in a parallel fashion similar to nerve growth factor and other neutrophins. Its dimerization is critical to fulfill its activity. When secreted by activated T cells, IL-17F can stimulate the production of other cytokines such as IL-6, IL-8 granulocyte colony-stimulating factor and, can stimulate cartilage matrix turnover. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and Inflammatory pathologies.
• Synonyms: mouse anti-IL-17F antibody, mouse anti-Interleukin-17F antibody, Cytokine ML-1, Interleukin-24
• Note: Anti-Human IL-17F antibody has been tested for use in IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user.