XDH Rabbit Polyclonal Antibody

CAT#: TA397062S

Xanthine Oxidase Antibody Biotin Conjugated

Product Images

Dot Blot results of Rabbit Anti-Xanthine Oxidase Antibody Biotin Conjugated. Dots are Xanthine Oxidase at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: Rabbit Anti-Xanthine Oxidase Antibody Biotin at 1µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP (p/n S000-03) at 1:40,000 for 30min at RT. Imaged with BioRad ChemiDoc, Chemi filter.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB: 1:250
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Xanthine Oxidase [Bovine Buttermilk]
• Specificity: Anti-Xanthine Oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk]. Cross reactivity against Xanthine Oxidase from other sources is unknown.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: Biotin
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Stability: Expiration date is one (1) year from date of receipt.
• Database Link:
  UniProt ID P80457
• Background: Anti-Xanthine Oxidase recognizes xanthine oxidase, an oxidoreductase. Xanthine oxidase catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid. Both of these reactions also generate the reactive oxygen species hydrogen peroxide. In addition, xanthine oxidase is involved in the catabolism of purines and metabolism of caffeine. Clinically, xanthine oxidase has been linked to gout, a condition in which uric acid accumulates in the kidneys and joints. Anti-Xanthine Oxidase antibody is ideal for investigators interested in Metabolism and Signal Transduction research.
• Synonyms: rabbit anti-Xanthine Oxidase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody
• Note: Anti-Xanthine Oxidase Biotin conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Xanthine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.