p27 KIP 1 (CDKN1B) Rabbit Polyclonal Antibody

CAT#: TA374584

CDKN1B/p27KIP1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 antibody (TA374584) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of C6 cells using CDKN1B/p27KIP1 Polyclonal Antibody (TA374584) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using CDKN1B/p27KIP1 Polyclonal Antibody (TA374584) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human CDKN1B/p27KIP1
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 22kDa
• Gene Name: cyclin-dependent kinase inhibitor 1B
• Database Link:
  Entrez Gene 1027 Human
  UniProt ID P46527
• Background: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).
• Synonyms: CDKN4; KIP1; MEN1B; MEN4; P27KIP1