ADAM12 Rabbit Polyclonal Antibody

CAT#: TA373252

ADAM12 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ADAM12 antibody (TA373252) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

• 

  Immunofluorescence analysis of C6 cells using ADAM12 antibody (TA373252) at dilution of 1:100. Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of HeLa cells using ADAM12 antibody (TA373252) at dilution of 1:100. Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of NIH/3T3 cells using ADAM12 antibody (TA373252) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:100 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 589-738 of human ADAM12 (NP_067673.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 80kDa/99kDa
• Gene Name: ADAM metallopeptidase domain 12
• Database Link:
  Entrez Gene 8038 Human
  UniProt ID O43184
• Background: This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form.
• Synonyms: MCMP; MCMPMltna; Meltrin-alpha; MLTN; MLTNA