p63 (TP63) Rabbit Polyclonal Antibody [Clone ID: Poly6190]

CAT#: TA327976

Rabbit Polyclonal anti-p63 (?N) Antibody

Product Images

Non-transfected 293 cell lysates (Lane 1) and p63 (deltaN) transfected 293 cell lysates (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p63 (deltaN) antibody (clone Poly6190). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.

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  Immunofluorescent microscope analysis of Hela cells using anti-p63 ΔN polyclonal antibody (poly6190) (red). a-tubulin (clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue).

Specifications

Product Data

• Clone Name: Poly6190
• Applications: IF, WB
• Recommended Dilution: WB, IF, IHC
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Modified peptide
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
• Purification: The antibody was purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52 kD, 45 kD
• Gene Name: tumor protein p63
• Database Link:
  NP_003713
  Entrez Gene 8626 Human
  UniProt ID Q9H3D4
• Background: p63 (ΔN) is a member of the p53 family. This protein has multiple isoforms TA p63-α, -β, γ, ΔN p63-α, -β, and -γ. The molecular weights of the p63 isoforms α=66 kD, β=52 kD, and γ=45 kD. This nuclear protein is essential for limb formation, epidermal morphogenesis, and epithelial stem cell regeneration. ΔN isoforms are antagonistic to p53; TA isoforms can transactivate p53 targets. The p63 ΔN isoforms repress TA isoforms; mutant p53 abrogates p63 transactivation. This protein interacts with p53, p73, and the various TA- and ΔN isoforms. The Poly6190 antibody recognizes human p63 ΔN isoforms and has been shown to be useful for Western blotting.
• Synonyms: AIS; B(p51A); B(p51B); EEC3; KET; LMS; NBP; OFC8; p40; p51; p53CP; p63; p73H; p73L; RHS; SHFM4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors