MAP2 Rabbit Polyclonal Antibody

CAT#: TA327383

MAP2 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using MAP2 antibody (TA327383) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 5min.

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  Immunofluorescence analysis of Mouse brain cells using MAP2 antibody (TA327383) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of Rat brain cells using MAP2 antibody (TA327383) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000
• Reactivities: Human, Rat, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human MAP2
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59 kDa
• Gene Name: microtubule associated protein 2
• Database Link:
  NP_002365
  Entrez Gene 17756 MouseEntrez Gene 25595 RatEntrez Gene 4133 Human
  UniProt ID P11137
• Background: Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites. Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623. Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability.
• Synonyms: MAP2A; MAP2B; MAP2C

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS