Nucleostemin (GNL3) Mouse Monoclonal Antibody [Clone ID: 3C12-H11]

CAT#: TA326394

Mouse monoclonal Hsp22 Antibody

Product Images

Western blot analysis of Hsp22 in rat tissue mix using a 1:1000 dilution of the antibody

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  IHC analysis of Hsp22, tested on mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute.

Specifications

Product Data

• Clone Name: 3C12-H11
• Applications: IHC, WB
• Recommended Dilution: WB: 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: His-tagged human recombinant hsp22
• Formulation: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: G protein nucleolar 3
• Database Link:
  NP_996562
  Entrez Gene 30877 MouseEntrez Gene 290556 RatEntrez Gene 26354 Human
  UniProt ID Q9BVP2
• Background: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta . Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 . HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs . The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy . Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) .
• Synonyms: C77032; E2IG3; NNP47; NS
• Note: Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency