Ubiquitin (UBB) Rabbit Polyclonal Antibody

CAT#: TA319488

Rabbit polyclonal Ubiquitin antibody

Product Images

Anti-Ubiquitin antibody, generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by WB against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at 1:500. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:1,000 - 1:5,000, WB: 1:200 - 1:1000, IHC: User Optimized
• Reactivities: Broad
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with ubiquitin coupled to rabbit IgG using glutaraldehyde.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ubiquitin B
• Database Link:
  NP_061828
  Entrez Gene 7314 Human
  UniProt ID P0CG47
• Synonyms: HEL-S-50
• Note: Ubiquitin (Ub) is a small, 76-residue, protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. Free Ub is a very compact and stable molecule that is easily refolded after being denatured. It is therefore recommended that for detection of free Ub on Westerns, the Tris-Tricine SDS-PAGE is used and nitrocellulose filters are autoclaved after the transfer and before blocking and addition of anti-Ub antibodies. The C-terminus of ubiquitin forms an isopeptide bond with the e-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein's function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction. Conjugation of ubiquitin (Ub) involves a three-step mechanism whereby specific enzymes (or enzyme complexes) activate and covalently link Ub to their substrates.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease