DKK1 Goat Polyclonal Antibody

CAT#: TA302464

Goat Polyclonal Antibody against DKK1

Product Images

TA302464 (1µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

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  TA302464 (10µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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  TA302464 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

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  TA302464 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Specifications

Product Data

• Applications: FC, IF, IHC, PEP-ELISA, WB
• Recommended Dilution: ELISA: 1:128,000. WB: 1-3µg/ml.
• Reactivities: Human (Expected from sequence similarity: Rat, Dog, Pig, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence CDHHQASNSSRLHT, from the C Terminus of the protein sequence according to NP_036374.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32296 Da
• Gene Name: dickkopf WNT signaling pathway inhibitor 1
• Database Link:
  NP_036374
  Entrez Gene 293897 RatEntrez Gene 609600 DogEntrez Gene 22943 Human
  UniProt ID O94907
• Background: This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq]
• Synonyms: DKK-1; SK

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Wnt signaling pathway