CRYAA2 Mouse Monoclonal Antibody [Clone ID: c9F2]

CAT#: SM6001S

CRYAA2 mouse monoclonal antibody, clone c9F2, Purified

Product Images

Western blot analysis: Mouse eye extracts and recombinant proteins (Crystallin-alpha A and B) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Crystallin alpha A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. This antibody does not show cross-activity about Crystallin alpha B. Lane A: Crystallin-alpha A recombinant protein, Lane B: Crystallin-alpha B recombinant protein, Lane C: Mouse eye lysates

Specifications

Product Data

• Clone Name: c9F2
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot (1:500 - 1:2,000), recommended starting dilution 1:1000.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human Crystallin alpha A (1-173aa) purified from E. coli
• Specificity: The antibody recognizes human , rat and mouse Crystallin-alpha A.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: crystallin alpha A2
• Database Link:
  Entrez Gene 102724652 Human
  UniProt ID A0A140G945
• Background: Crystallins alpha are composed of two gene products; alpha A and alpha B, for acidic and basic, respectively. crystallins alpha can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha A and alpha B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The expression of alpha-A is preferentially restricted to the lens cell.
• Synonyms: CRYAA, HSPB4, Heat shock protein beta-4