CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: AT10]
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Specifications
Product Data | |
Clone Name | AT10 |
Applications | ELISA, FC, FN, IHC, IP |
Recommended Dilution | ELISA. Functional assays. Immunoprecipitation: 20 µg/ml. Flow Cytometry: Use 10 µl of 20 µg/ml diluted CD32 antibody to label 1x106 cells or 100 µl whole blood. Immunohistochemistry on Frozen Sections: 1/500-1/1000. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. It is recommended to use acetone fixation for frozen sections. Histology Positive Control tissue: Lymph node. Not suitable for Paraffin Sections. |
Reactivities | Canine, Human, Monkey, Rhesus Monkey |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | K562 cell line |
Specificity | This Monoclonal antibody AT10 recognises the Human CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma RII (Ref. 4). |
Formulation | PBS without preservatives State: Low Endotoxin State: Liquid purified IgG fraction |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 20°C only. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | Fc fragment of IgG receptor IIa |
Database Link | |
Background | CD32 antigen is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. |
Synonyms | Fc-gamma RII-a, Fc-gamma-RIIa, CDw32, FCGR2A, FCG2, FCGR2A1, IGFR2 |
Note | Endotoxin Level: < 0.01EU/µg |
Reference Data |
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