CD3E Mouse Monoclonal Antibody [Clone ID: UCHT1]

CAT#: SM1057R

CD3E mouse monoclonal antibody, clone UCHT1, PE

Product Images

Staining of human peripheral blood lymphocytes with Mouse Anti Human CD3-RPE.

Specifications

Product Data

• Clone Name: UCHT1
• Applications: FC
• Recommended Dilution: Flow cytometry.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
• Specificity: This antibody recognises CD3, a 19 kD cell surface protein which is expressed on more than 95% of circulating peripheral blood T-cells.
• Formulation: PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with distilled water.
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD3e molecule
• Database Link:
  Entrez Gene 916 Human
  UniProt ID P07766
• Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
• Synonyms: T3/Leu-4