CPB1 Rabbit Polyclonal Antibody

CAT#: R1071BS

CPB1 rabbit polyclonal antibody, Biotin, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/500-1/3,000
  ELISA: 1/5,000-1/25,000
  This product has been assayed against 1.0 µg of Carboxypeptidase B [porcine pancreas] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/3,000 of the reconstitution concentration is suggested.
• Reactivities: Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Carboxypeptidase B from porcine pancreas
• Specificity: This product detects porcine Carboxypeptidase B. Cross reactivity against Carboxypeptidase B from other tissues and species is unknown. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit Serum as well as purified and partially purified Carboxypeptidase B [porcine pancreas].
  
  
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/m BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P09955
• Background: Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates.
• Synonyms: CPB1, CPB, PCPB, Pancreas-specific protein, PASP