IL22 Rabbit Polyclonal Antibody

CAT#: PP1224B1

IL22 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human IL-22 by direct ELISA (using 100 µl/well) a concentration of ~1.0 µg/ml of this antibody is required. The Biotinylated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-22.
  Sandwich ELISA: To detect Human IL-22 by Sandwich ELISA (using 100 µl/well) a concentration of 0.25-1.0 µg/ml of this antibody is required. The Biotinylated antibody, in conjuction with anti-Human IL22 (PP1224P1 or PP1224P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-22.
  Western blot: To detect Human IL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-22 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human IL-22 (hIL-22).
• Specificity: Recognizes Human Interleukin-22 (IL-22).
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore with sterile PBS solution containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C.
  Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interleukin 22
• Database Link:
  Entrez Gene 50616 Human
  UniProt ID Q9GZX6
• Background: IL22 is a cytokine structurally related to IL10 and is upregulated in activated T cells. IL22 signals through a receptor complex that includes CRF2-4 and a new member of the class II cytokine receptor family (also known as the interferon receptor family), IL22R. CRF2-4 has previously been demonstrated to be a functional component of the IL-10 signaling complex.
  IL22 is able to suppress IL4 production; this may have therapeutic potential, particularly in the treatment of asthma.
• Synonyms: IL-22, ILTIF, Cytokine Zcyto18

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway