LTA Rabbit Polyclonal Antibody

CAT#: PP1072B1

LTA rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA:
  (Direct): To detect hTNF-β by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-β.
  (Sandwich): To detect hTNF-β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-β (PP1072P1/PP1072P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-β.
  Western Blot: To detect hTNF-β by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-β is 1.5- 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hTNF-beta.
• Specificity: This antibody reacts with Tumor Necrosis Factor beta.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: lymphotoxin alpha
• Database Link:
  Entrez Gene 4049 Human
  UniProt ID P01374
• Synonyms: TNFB, TNFSF1, LTA, LT-alpha
• Note: Centrifuge vial prior to opening!