DEF6 Mouse Monoclonal Antibody [Clone ID: OTI1E3]

CAT#: CF505321

Carrier-free (BSA/glycerol-free) DEF6 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF6 (RC217877, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF6. Positive lysates LY411815 (100ug) and LC411815 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-DEF6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-DEF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505321)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DEF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505321)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DEF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505321)

• 

  Anti-DEF6 mouse monoclonal antibody (TA505321) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF6 (RC217877).

Show all 6 images

Specifications

Product Data

• Clone Name: OTI1E3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DEF6(NP_071330) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.7 kDa
• Gene Name: DEF6 guanine nucleotide exchange factor
• Database Link:
  NP_071330
  Entrez Gene 23853 MouseEntrez Gene 309642 RatEntrez Gene 608163 DogEntrez Gene 718845 MonkeyEntrez Gene 50619 Human
  UniProt ID Q9H4E7
• Background: DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]). [supplied by OMIM]
• Synonyms: IBP; SLAT; SWAP70L

Reference Data

• Protein Families: Druggable Genome