Plakophilin 3 (PKP3) Mouse Monoclonal Antibody [Clone ID: 23E3/4]

CAT#: BM6031P

Plakophilin 3 (PKP3) mouse monoclonal antibody, clone 23E3/4, Purified

Product Images

Immunohistochemical detection of Plakophilin-3 in Paraffin Sections of Formalin-Fixed Human skin using monoclonal antibody 23E3/4. SC = Stratum Corneum Ep = Epidermis D = Dermis

Specifications

Product Data

• Clone Name: 23E3/4
• Applications: IF, IHC, WB
• Recommended Dilution: Western blot (Detection).
  Immunoprecipitation
  Immunocytochemistry (Methanol-and paraformaldehyde-fixed cells).
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Embedded Sections.
  For optimal results, fixed cells and frozen tissues should be treated with 0.2% Triton-X 100 for, respectively, 5 min and 15 min prior to incubation with primary antibody.
  Recommended Dilutions: 1/25-1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/1000 for Immunoblotting.
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to amino acid residues 570-589 of Human Plakophilin-3 coupled to Keyhole Limpet Hemocyanin via an additional cysteine residue at the N-terminus (CFTPQSRRLRELPLAADALTF).
• Specificity: This antibody 23E3/4 reacts with an epitope located between residues 570-589 in Human Plakophilin-3.
• Formulation: PBS with 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: plakophilin 3
• Database Link:
  Entrez Gene 11187 Human
  UniProt ID Q9Y446
• Background: Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin- 3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin- 3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.
• Synonyms: Plakophilin-3