Neurofilament (NEFM) Mouse Monoclonal Antibody [Clone ID: RNF403]

CAT#: BM6011P

Neurofilament (NEFM) mouse monoclonal antibody, clone RNF403, Purified

Product Images

Immunofluorescence staining of Rat brain Frozen Sections (Acetone fixed) with a 1/100 dilution of (Clone RNF403) directed against the 160kD component of neurofilaments.

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  Immunofluorescence staining of Rat brain Frozen Sections (acetone fixed) with a 1/100 dilution of (Clone RNF403) directed against the 160kD component of neurofilaments.

Specifications

Product Data

• Clone Name: RNF403
• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunocytochemistry/Immunofluorescence.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin-Embedded Tissues.
  Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and for 1/100-1/500 for Immunoblotting.
• Reactivities: Hamster, Human, Monkey, Rat, Xenopus
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Neurofilament preparation of calf brain tissue.
• Specificity: RNF403 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: neurofilament, medium polypeptide
• Database Link:
  Entrez Gene 4741 Human
  UniProt ID P07197
• Background: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
• Synonyms: Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker)