Cingulin (CGN) Mouse Monoclonal Antibody [Clone ID: 139.3.4]

CAT#: BM5098

Cingulin (CGN) mouse monoclonal antibody, clone 139.3.4, Supernatant

Specifications

Product Data

• Clone Name: 139.3.4
• Applications: IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunohistochemistry on Frozen and Paraffin Sections (only after microwave treatment): Ready-to-use.
  On cultured cell fixation with paraformaldehyde is recommended for optimal staining.
• Reactivities: Bovine, Human, Mouse, Xenopus
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (aa. 4-24)
• Specificity: This monoclonal antibody reacts specifically with Cingulin (a 150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas), of transitional epithelia (e.g. bladder epithelium), and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina).
  Negative with endothelia of vessels and capillaries (e.g. in heart and brain).
  In contrast to Symplekin, using Monoclonal antibody 139.4 Cingulin has been localized exclusively to the tight junction complex.
  Tested Reactivities on Cultured Cell Lines: Carcinoma cell lines (e.g. MCF-7, Caco-2, PLC, A-431), SV80 (fibroblasts), Glioma; MDBK, MDCK, BMGE.
• Formulation: State: Supernatant
  State: Liquid Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: cingulin
• Database Link:
  Entrez Gene 57530 Human
  UniProt ID Q9P2M7
• Background: Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to that of symplekin, another junctional protein).
• Synonyms: CGN, KIAA1319