MAP2 (2a + 2b) Mouse Monoclonal Antibody [Clone ID: AP20]

CAT#: BM2472

MAP2 (2a + 2b) mouse monoclonal antibody, clone AP20, Purified

Product Images

Formalin-fixed, paraffin-embedded human brain stained with Microtubule-Associated Protein 2a, b Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of the neurons and dendrites.

Specifications

Product Data

• Clone Name: AP20
• Applications: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1/500.
  Immunofluorescence: 1/500.
  Western Blotting: 1-2 µg/ml for 2hrs at RT.
  
  
  
• Reactivities: Bovine, Chicken, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bovine brain Microtubule Associated Protein 2.
• Specificity: Thisantibody recognizes (Mr300 kDa) MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from Human, Rat, Mouse and Chicken brains. BM2472 reacts with the High Molecular Weight forms (2a & 2b) of MAP-2 but not with the Low Molecular Weight (70 kDa) form (2c).
  It reacts with dendrites and cell bodies of neurons.
• Formulation: 0.01M PBS pH 7.4 containing 1% BSA as stabilizer and 0.09% Sodium Aazide as a preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluited at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: microtubule associated protein 2
• Database Link:
  Entrez Gene 4133 Human
  UniProt ID P11137
• Background: MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.
• Synonyms: Microtubule-associated protein 2, MAP2, Neuronal Marker