CD42b / GPIbA Mouse Monoclonal Antibody [Clone ID: SZ2]

CAT#: BM2320

CD42b / GPIbA mouse monoclonal antibody, clone SZ2, Azide Free

Specifications

Product Data

• Clone Name: SZ2
• Applications: FC, FN, IHC
• Recommended Dilution: Studies of platelet functions.
  Only suitable on frozen sections or cell smears.
  May be used in Western blot.
  Fluorescence Microscopy or Flow Cytometry: 2 µg/5x10e6 platelets/test.
  Immunohistochemistry: Working dilution 1:50 to 1:100.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human washed platelets.
• Specificity: The molecular weight of the recognized antigen is 170 kDa.
  Reacts with Glycoprotein Ib on Megakaryocytes and platelets.
  Inhibits the Ristocetin-dependent binding of Von Willebrand factor to platelets and Ristocetin induced platelet agglutination.
  Inhibits platelet aggregation induced by Type I Collagen and platelet activating factor (PAF).
  Immunoprecipitates the components of Glycoprotein Ib complex.
• Formulation: PBS containing 1 mg/ml BSA without preservatives.
  State: Azide Free
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 1 ml distilled water.
• Concentration: lot specific
• Purification: Protein A chromatography.
• Conjugation: Unconjugated
• Storage: Store the reconstituted antibody at 2-8°C for one month (add 0.09% Sodium Azide) or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glycoprotein Ib platelet alpha subunit
• Database Link:
  Entrez Gene 2811 Human
  UniProt ID P07359
• Background: CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.
• Synonyms: Platelet glycoprotein Ib alpha chain, Glycoprotein Ibalpha, GP-Ib alpha, GPIb-alpha