Periostin (POSTN) (C-term) Rabbit Polyclonal Antibody

CAT#: AP53392PU-N

Periostin (POSTN) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Periostin Antibody (C-term) in MCF-7, A375 cell line lysates (35ug/lane). This demonstrates the POSTN antibody detected the POSTN protein (arrow).

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  Formalin fixed and paraffin embedded human uterus tissue reacted with Periostin Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of Periostin Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution:

  Western Blot: ~ 1/1000.
  Immunofluorescence: 1/10-1/50.
  Flow Cytometry: 1/25.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 687-716 amino acids from the C-terminal region of human Periostin.
• Specificity: This antibody recognizes Human Periostin (C-term).
  Cellular Location: Golgi apparatus
  Tissue Location: Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast.
  Up-regulated in epithelial ovarian tumors; not expressed in normal ovaries.
  Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor.
  Overexpressed in breast cancers.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A chromatogrphy, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: periostin
• Database Link:
  Entrez Gene 10631 Human
  UniProt ID Q15063
• Background: POSTN binds to heparin. It induces cell attachment and spreading and plays a role in cell adhesion and may play a role in extracellular matrix mineralization.
• Synonyms: POSTN, OSF2, OSF-2, PN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein