IL31 (N-term) Rabbit Polyclonal Antibody

CAT#: AP52198PU-N

IL31 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of IL31 Antibody (N-term) in mouse spleen tissue lysates (35ug/lane). IL31 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human testis tissue reacted with IL31 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Flow cytometric analysis of WiDr cells using IL31 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 19~49 amino acids from the N-terminal region of Human IL31
• Specificity: This antibody recognizes Interleukin 31 (N-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 18205 Da
• Gene Name: interleukin 31
• Database Link:
  Entrez Gene 76399 MouseEntrez Gene 386653 Human
  UniProt ID Q6EBC2
• Background: IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma.
• Synonyms: IL-31

Reference Data

• Protein Families: Secreted Protein, Transmembrane