IL12RB2 (C-term) Rabbit Polyclonal Antibody

CAT#: AP52184PU-N

IL12RB2 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in MDA-MB435 cell line lysates (35ug/lane) using IL12RB2 antibody. This demonstrates the IL12RB2 antibody detected the IL12RB2 protein (arrow).

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  Immunohistochemistry analysis in human tonsils tissue (formalin-fixed, paraffin-embedded) using IL12RB2 antibody. (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12RB2 antibody for IHC. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram) using IL12RB2 antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  This antibody is suitable for flow cytometry, immunohistochemistry on paraffin sections and Western blotting.
  The suggested dilution is:
  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on paraffin sections: 1/50-1/100.
  Western blotting: 1/100-1/500.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 759-788aa) of human IL12RB2.
• Specificity: This antibody detects human IL12RB2 at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium Azide.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a protein A column; followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: interleukin 12 receptor subunit beta 2
• Database Link:
  Entrez Gene 3595 Human
  UniProt ID Q99665
• Background: The protein encoded by the IL12RB2 gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense.
• Synonyms: IL-12 receptor beta-2, IL-12R-beta-2
• Note: Molecular Weight: 97135 Da

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway