CD1E (Center) Rabbit Polyclonal Antibody

CAT#: AP50840PU-N

CD1E (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

CD1E Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CD1E antibody detected the CD1E protein (arrow).

• 

  CD1E Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD1E Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

• 

  Confocal immunofluorescent analysis of CD1E Antibody (Center) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

• 

  CD1E Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow Cytometry: 1/10 - 1/50.
  Immunofluorescence: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human CD1E
• Specificity: This antibody reacts to CD1E.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 43626
• Gene Name: CD1e molecule
• Database Link:
  Entrez Gene 913 Human
  UniProt ID P15812
• Background: CD1E encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members.
• Synonyms: R2G1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage