Glypican 4 (GPC4) Mouse Monoclonal Antibody [Clone ID: AT51E3]

CAT#: AP33507PU-S

Glypican 4 (GPC4) mouse monoclonal antibody, clone AT51E3, Purified

Product Images

ICC/IF analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPC4 antibody (1/200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

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  Flow cytometry analysis of GPC4 in 293T cell line, staining at 2-5 µg for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

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  The Recombinant Human GPC4 (50ng) and Cell lysate (40 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPC4 anibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. Recombinant Human GPC4 Lane 2. Ramos cell lysate

Specifications

Product Data

• Clone Name: AT51E3
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1/1000).
  Flow Cytometry (1/200).
  Immunofluorescence (1/200).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Human GPC4 (401-529 aa) purified from E. coli.
• Specificity: The antibody recognizes Human GPC4. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glypican 4
• Database Link:
  Entrez Gene 2239 Human
  UniProt ID O75487
• Background: The glypican (Gpc) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the stage of intiation of neural tube closure, suggesting that glypican-4 is unlikely to play an essential role in convergent extension in the mouse.
• Synonyms: Glypican 4, K-glypican

Reference Data

• Protein Families: Druggable Genome