ALB Rabbit Polyclonal Antibody

CAT#: AP32991SU-N

ALB rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP, R
• Recommended Dilution: As precipitating antiserum to identify or measure duck albumin by a variety of Immunodiffusion techniques, including immunoelectrophoresis, Single and Double Radial immunodiffusion (Mancini, Ouchterlony) and Electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or Immunochemistry, but this does not exclude such use if proper controls are included.
  Recommended Working Dilutions:
  Immunoelectrophoresis: Use 2 µl serum, plasma or equivalent against 120 µl antiserum.
  Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  Single Radial Immunodiffusion and Electroimmunodiffusion: Use 0.5 to 1.0% antiserum in the agar gel.
  Antibody Titre: Precipitin titre not less than 1/64 when tested against normal Bovine Serum in agar block immunodiffusion titration.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified albumin isolated from Bovine serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The defined antibody specificity is directed to albumin as tested against bovine sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against bovine serum and the purified albumin.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in radial immunodiffusion with the following results:
  (-) Species: Cat, Chicken, Dog, Duck, Goat, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Pigeon, Rabbit.
  (+) Species: Rat.
  (±) Species: Swine.
• Formulation: State: Serum
  State: Lyophilized (delipidated, heat inactivated) stable whole antiserum
  Preservative: None
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: Total protein and IgG concentrations in the antiserum are comparable to those in pooled Sheep serum. No foreign proteins added.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 280717 Bovine
  UniProt ID P02769
• Synonyms: ALB, BSA, HSA, Serum Albumin
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.