Creatine kinase M type (CKM) (N-term) Goat Polyclonal Antibody

CAT#: AP32737PU-N

Creatine kinase M type (CKM) (N-term) goat polyclonal antibody, Aff - Purified

Product Images

CKMM Antibody staining of Human (A, C) and Mouse (B, D) Skeletal Muscle (A, B) and Heart (C, D) lysates at 0.01ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 0.01-0.3 µg/ml. Approx 45kDa band observed in Human and Mouse Skeletal Muscle and Heart lysates (calculated MW of 43.1kDa according to NP_001815.2).
• Reactivities: Canine, Human, Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the N-Terminus of the protein sequence according to NP_001815.2.
• Specificity: This antibody recognizes creatine kinase M-type (CKMM) at N-term.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: creatine kinase, M-type
• Database Link:
  Entrez Gene 1158 Human
  UniProt ID P06732
• Synonyms: Creatine kinase M-type, Creatine kinase M chain, M-CK, CKM, Creatine kinase MM