Nucleoside phosphorylase (PNP) (142-153) Goat Polyclonal Antibody

CAT#: AP32724PU-N

Nucleoside phosphorylase (PNP) (142-153) goat polyclonal antibody, Aff - Purified

Product Images

PNP Antibody staining of Human Liver lysate at 0.1ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 0.03-0.1 µg/ml. Approx 30kDa band observed in lysates of Human Liver, Erythrocytes and of cell lines Daudi, Jurkat and K562 (calculated MW of 32.1kDa according to NP_000261.2).
  Immunohistochemistry on Paraffin Sections: 5 µg/ml in Paraffin embedded Human Tonsil and Kidney.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000261.2.
• Specificity: This antibody recognizes Human PNP (aa142-153).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: purine nucleoside phosphorylase
• Database Link:
  Entrez Gene 4860 Human
  UniProt ID P00491
• Synonyms: PNP, PNP1, Inosine Phosphorylase